Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/91
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dc.contributor.authorIlori, Matthew O.-
dc.contributor.authorAmund, Olukayode O.-
dc.contributor.authorOmidiji, O.-
dc.date.accessioned2019-06-20T10:52:34Z-
dc.date.available2019-06-20T10:52:34Z-
dc.date.issued1997-10-01-
dc.identifier.citationIlori, M. O, Amund, O.O. and Omidiji, O. (1997) Purification and Properties of an A-Amylase Produced by a Cassava-Fermenting Strain of Micrococcus Luteus. Folia Microbiologica 42 (5)en_US
dc.identifier.uri10.1007/BF02826551-
dc.identifier.urihttp://165.22.87.194:8080/jspui/handle/20.500.12398/91-
dc.description.abstractAn extracellular α-amylase produced by a cassava-fermenting strain of Micrococcus luteus was purified 26-fold by gel filtration and ion-exchange chromatography. The molar mass was estimated to be approximately 56 kDa. The optimum temperature of the enzyme was 30oC, optimum pH 6.0 and optimum substrate concentration was 0.6 % (w/v). Treatment of the enzyme at 70 oC for 10 min resulted in 70 % loss of activity. The activation energy was determined to be 34.8 kj/mol. The activity of the enzyme was enhanced by Mg2+, Ca2+, K+, Na+ and inhibited by EDTA, KCN and citric acid. The enzyme may find some application in local food processing.en_US
dc.language.isoenen_US
dc.publisherSpringer Netherlandsen_US
dc.subjectFermentationen_US
dc.subjectMicro organismsen_US
dc.titlePurification and properties of an α-amylase produced by a cassava-fermenting strain of Micrococcus luteusen_US
dc.typeArticleen_US
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