EUSpace Collection:
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/25
2023-08-23T23:40:01ZAnalysis of Decomposing Wood Wastes in the Lagos Lagoon using Terminal-Restriction Fragment Length Polymorphism and Illumina Sequencing Platform
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1402
Title: Analysis of Decomposing Wood Wastes in the Lagos Lagoon using Terminal-Restriction Fragment Length Polymorphism and Illumina Sequencing Platform
Authors: Buraimoh, O. M.; Adewumi, G. A.; Akinyemi, N. M.; Amund, O. O.; Ilori, M. O.; Michel Jr., F. C.
Abstract: Indiscriminate disposal of wood shavings and sawdust into the Lagos lagoon usually constitute
environmental hazard and varying degree of threats to marine biotic communities. In this study we applied terminalrestriction fragment length polymorphism (T-RFLP) and illumina sequencing of 16S ribosomal RNA gene, to describe
the microbial ecology of decomposing wood wastes in the Lagos lagoon, Nigeria. The terminal restriction fragments
(TRFs) generated corresponds to over 100 bacterial genera and numerous uncultured bacterial clones. Phylogenetic
analysis of the TRFs of 16S rRNA gene sequences and cultured bacterial sequences established genetic relatedness
and divergent relationships. T-RFLP in combination with illumina sequencing platform identified bacterial species
(Acinetobacter sp., Clostridium sp., Planctomyces sp., Escherichia coli, Pantoea agglomerans, Dyella japonica,
Ochrobactrum sp., Prevotella sp., Runella slithyformis, Enterobacter aerogenes, Gordonia amicalis, Klebsiella sp.,
Thalassospira sp. and Serratia sp.) not previously accounted for in culture-dependent analysis. Bacterial strains such
as Ruminococcus flavefaciens, Fibrobacter succinogenes, Simiduia agarivorans, Microbulbifer sp. and Cellulomonas
fimi further identified in illumina sequences were found to be involved in the biodegradation of lignin and lignin
derivatives. Hence, understanding of the diverse autochthonous microbiota of decomposing wood wastes in the Lagos
lagoon is essential in the development of effective biotechnology programme, to tackling the menace of sawdust
pollution.
Description: Staff Publication2022-06-01T00:00:00ZNitrilase gene detection and nitrile metabolism in two bacterial strains associated with waste streams in Lagos, Nigeria
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1401
Title: Nitrilase gene detection and nitrile metabolism in two bacterial strains associated with waste streams in Lagos, Nigeria
Authors: Ogunyemi, Adewale Kayode; Buraimoh, Olanike Maria; Ogunyemi, Bukola Caroline; Samuel, Titilola Aderonke; Ilori, Matthew Olusoji; Amund, Olukayode Oladipo
Abstract: Background: The use of nitrile compounds is usually high, particularly in chemical industries, which calls for serious concern because of their relevance to the environment. The essential role of nitrilases in the bioremediation of
harmful nitriles from environmental wastes cannot be overemphasized. The study aimed to unveil the biodegradative
potentials of bacterial strains associated with the degradation of nitrile pollutants.
Methods: Bacterial strains capable of utilizing glutaronitrile as the sole source of carbon and nitrogen were isolated
from solid waste leachates by a selective enrichment culture technique. The test organisms were grown in mineral
salts medium (MSM), and the metabolic products were determined using gas chromatography-fame ionization
detection (GC-FID). The nitrilase gene was amplifed by polymerase chain reaction (PCR) and by using appropriate
primers.
Results: The growth studies showed that the test organisms grew on the two nitriles. The doubling times of 12.16
d and 9.46 d (specifc growth rate, µ=0.082 d−1
, 0.106 d−1
) were obtained for each pure culture of Bacillus sp. srain
WOD8 and Corynebacterium sp. srain WOIS2 on glutaronitrile (as single substrate), respectively. While the same strains
had doubling times of 11.11 d and 10.00 d (µ=0.090 d−1
, 0.100 d−1
) on benzonitrile (as single substrate). However,
the mixed culture (comprising the two strains) had doubling times of 7.40 d and 7.75 d (µ=0.135 d−1
, 0.129 d−1
)
on glutaronitrile (as single and mixed substrates), respectively. While doubling times of 8.09 d and 8.71 d (µ=0.124
d−1
, 0.115 d−1
) were obtained for the same mixed culture on benzonitrile (as single and mixed substrates). Based on
gas chromatographic analysis, the residual glutaronitrile concentrations at day 16 for strains WOD8 and WOIS2 were
35.77 g L−1
(72.2%) and 9.30 g L−1
(92.5%), respectively, whereas the residual benzonitrile concentrations for the same
strains were 27.39 g L−1
(78.8%) and 13.79 g L−1
(89.2%), respectively. For the mixed culture, residual glutaronitrile and
benzonitrile concentrations at day 16 were 13.40 g L−1
(88.5%) and 10.42 g L−1
(91.5%), respectively, whereas for the
mixed substrates (glutaronitrile and benzonitrile), 7.21 g L−1
(91.7%) and 4.80 g L−1
(94.2%) of residual glutaronitrile
and benzonitrile concentrations were obtained by the same consortium. The gene for nitrilase involved in nitrile degradation was detected in the genome of the bacterial strains. The amplifed nitrilase gene gave PCR products of sizes
1400 bp and 1000 bp, as expected for strains WOD8 and WOIS2, respectively. 4-Cyanobutyric acid (4CBA), glutaric
acid (GA), and benzoic acid (BA) were obtained as metabolites following nitrile degradation in vitro.Conclusion: These results revealed that strains WOD8, WOIS2 and the mixed culture (consisting of the
two strains) have proven to have the capacity to metabolize nitriles (glutaronitrile and benzonitrile) as the carbon and
nitrogen sources. However, the mixed culture had higher nitrile degradation rate as compared to each pure culture of
the two test organisms. These results also provide insight into the evolutionary genetic origin of a nitrilase gene that
encodes an enzyme that catalyzes nitrile degradation in these strains. Hence, the bacterial strains that harbor this kind
of gene may be used as promising biological agents for the remediation of sites polluted with nitriles, thereby opening new perspectives for encouraging data for a bioremediation bioprocess
Description: Staff Publication2022-05-01T00:00:00ZA global metagenomic map of urban microbiomes and antimicrobial resistance
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1353
Title: A global metagenomic map of urban microbiomes and antimicrobial resistance
Authors: Osuolale, Olayinka O.; Danko, David; Bezdan, Daniela; et al
Abstract: We present a global atlas of 4,728 metagenomic samples from mass-transit systems in 60 cities over 3 years,
representing the first systematic, worldwide catalog of the urban microbial ecosystem. This atlas provides an
annotated, geospatial profile of microbial strains, functional characteristics, antimicrobial resistance (AMR)
markers, and genetic elements, including 10,928 viruses, 1,302 bacteria, 2 archaea, and 838,532 CRISPR arrays not found in reference databases. We identified 4,246 known species of urban microorganisms and a
consistent set of 31 species found in 97% of samples that were distinct from human commensal organisms.
Profiles of AMR genes varied widely in type and density across cities. Cities showed distinct microbial taxonomic signatures that were driven by climate and geographic differences. These results constitute a highresolution global metagenomic atlas that enables discovery of organisms and genes, highlights potential
public health and forensic applications, and provides a culture-independent view of AMR burden in cities.
Description: Staff Publication2021-05-26T00:00:00ZAntioxidant activities and glycemic indices of ice creams enriched with orange (Citrus sinensis) and shaddock (Citrus maxima) peels and effects on rat lipid profiles
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1316
Title: Antioxidant activities and glycemic indices of ice creams enriched with orange (Citrus sinensis) and shaddock (Citrus maxima) peels and effects on rat lipid profiles
Authors: Ademosun, Ayokunle Olubode; Oboh, Ganiyu; Ajeigbe, Olufunke Florence
Abstract: This study evaluated the effects of ice creams produced from blends of orange (Citrus
sinensis) and shaddock (Citrus maxima) peels on the blood lipid profile, glycemic index,
and antioxidant indices in the liver and heart of rats. Formulated ice cream was produced
at a different proportion of citrus (orange and shaddock) blends and fed to
rats for 28 days. The result showed that the formulated ice cream enriched with citrus
peels blends caused a significant increase in high-density
lipoprotein-cholesterol
level in the plasma and antioxidant status in the liver and heart homogenates, decreased
the glycemic index, concentration of total cholesterol, triglycerides, and
low-density
lipoprotein in the plasma as against rats fed on plain and commercial ice
creams. To conclude, the use of ice creams from blends of orange and shaddock peels
could serve as a functional food for weight reduction, glycemic index, management
of lipid-related
diseases, and prevention of oxidative stress-related
complications in
the liver and heart.
Practical applications
The consumption of ice creams has increased in many parts of the world. However,
there have been limited efforts aimed at improving the medicinal properties of frozen
dairy products. Hence, these ice creams could be produced on a large scale under
regulated condition since they have improved medicinal properties which would be
a good option for preventing/combating degenerative conditions and their related
complications.
Description: Staff Publication2021-05-01T00:00:00Z