Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1228
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dc.contributor.authorObayori, Oluwafemi Sunday-
dc.contributor.authorSalam, Lateef B.-
dc.contributor.authorOgunmakinwa, Olubusola Olayinka-
dc.contributor.authorOgunleye, Moyosore Adedolamu-
dc.date.accessioned2021-09-09T11:05:19Z-
dc.date.available2021-09-09T11:05:19Z-
dc.date.issued2017-12-
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1228-
dc.descriptionStaff Publicationen_US
dc.description.abstractIntroduction: Poultry manure and cow dung have been established as potential material for the bioremediation of petroleum polluted sites, with emphasis on nutrient addition. Aim: Our aim was to isolate from poultry droppings and cow dung bacteria with ability to degrade petroleum hydrocarbons. Materials and Methods: Bacteria were isolated from poultry droppings and cow dung by continuous enrichment and vapor transfer techniques. Petroleum utilization by each isolate was confirmed in carbon free medium containing Escravos crude oil (1%). The two best isolates were selected for further study. Isolates were identified by Analytical Profile Index (API). Antibiotic sensitivity was determined by multidisc (Abitek Multidisc, UK). Growth was assayed in broth culture by plate count. Residual oil was determined by Gas Chromatography equipped with Flame Ionisation detector (GC-FID). Results: The isolates were putatively identified as Pseudomonas putida (MP2) and Pseudomonas sp. (MC4). Both isolates were susceptible to ciprofloxacin and chloramphenicol and tarivid. They resisted amoxicillin and gentamycin, augmentin, sparfloxacin and septrin. The growth rates were 0.17 and 0.23/day for strains MP2 and MC4 respectively, while the organisms degraded 88.39% and 89.06 % of crude oil respectively in 20 days. Aliphathic hydrocarbons in the range C11 to C22 were mostly reduced to less than 20%, while C22 – C26 disappeared completely within the same period in both cases. Conclusion: Bacteria capable of extensive degradation of Escravos crude oil were isolated from poultry droppings and cow dung. Such isolates could be veritable candidates for bioaugmentation of hydrocarbon polluted environmental compartments.en_US
dc.language.isoenen_US
dc.publisherLASU Journal of Research and Review in Scienceen_US
dc.subjectBiodegradation ;en_US
dc.subjectBioremediation ;en_US
dc.subjectPoultry droppings ;en_US
dc.subjectCow dung ;en_US
dc.subjectCrude oil ;en_US
dc.subjectGas chromatographyen_US
dc.titleDegradation of crude oil (Escravos light) by Pseudomonas strains isolated from poultry droppings and cow dung.en_US
dc.typeArticleen_US
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