Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1239
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dc.contributor.authorSalam, Lateef B.-
dc.contributor.authorObayori, Oluwafemi Sunday-
dc.date.accessioned2021-09-13T10:35:24Z-
dc.date.available2021-09-13T10:35:24Z-
dc.date.issued2020-
dc.identifier.urihttps://doi.org/10.1186/s43141-020-00089-9-
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1239-
dc.descriptionStaff Publicationen_US
dc.description.abstractBackground: Soils polluted with animal charcoal from skin and hide cottage industries harbour extremely toxic and carcinogenic hydrocarbon pollutants and thus require a bio-based eco-friendly strategy for their depuration. The effects of carbon-free mineral medium (CFMM) amendment on hydrocarbon degradation and microbial community structure and function in an animal charcoal-polluted soil was monitored for 6 weeks in field moist microcosms consisting of CFMM-treated soil (FN4) and an untreated control (FN1). Hydrocarbon degradation was monitored using gas chromatography-flame ionization detector (GC-FID), and changes in microbial community structure were monitored using Kraken, while functional annotation of putative open reading frames (ORFs) was done using KEGG KofamKOALA and NCBI’s conserved domain database (CDD). Results: Gas chromatographic analysis of hydrocarbon fractions revealed the removal of 84.02% and 82.38% aliphatic and 70.09% and 70.14% aromatic fractions in FN4 and FN1 microcosms in 42 days. Shotgun metagenomic analysis of the two metagenomes revealed a remarkable shift in the microbial community structure. In the FN4 metagenome, 92.97% of the population belong to the phylum Firmicutes and its dominant representative genera Anoxybacillus (64.58%), Bacillus (21.47%) and Solibacillus (2.39%). In untreated FN1 metagenome, the phyla Proteobacteria (56.12%), Actinobacteria (23.79%) and Firmicutes (11.20%), and the genera Xanthobacter (9.73%), Rhizobium (7.49%) and Corynebacterium (7.35%), were preponderant. Functional annotation of putative ORFs from the two metagenomes revealed the detection of degradation genes for aromatic hydrocarbons, benzoate, xylene, chlorocyclohexane/chlorobenzene, toluene and several others in FN1 metagenome. In the FN4 metagenome, only seven hydrocarbon degradation genes were detected. Conclusion: This study revealed that though CFMM amendment slightly increases the rate of hydrocarbon degradation, it negatively impacts the structural and functional properties of the animal charcoal-polluted soil. It also revealed that intrinsic bioremediation of the polluted soil could be enhanced via addition of water and aerationen_US
dc.language.isoenen_US
dc.publisherJournal of Genetic Engineering and Biotechnologyen_US
dc.subjectAnimal charcoal-polluted soil,en_US
dc.subjectCarbon-free mineral medium,en_US
dc.subjectHydrocarbon degradation,en_US
dc.subjectIllumina shotgun sequencing,en_US
dc.subjectMicrobial Community Structure,en_US
dc.subjectSoil microcosmen_US
dc.titleRemarkable shift in structural and functional properties of an animal charcoalpolluted soil accentuated by inorganic nutrient amendmenten_US
dc.typeArticleen_US
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