Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/815
Title: Application of Fenton reaction for nanomolar determination of hydrogen peroxide in seawater
Authors: Olasehinde, Emmanuel F.
Makino, Shinya
Kondo, Hiroaki
Takeda, Kazuhiko
Sakugawa, Hiroshi
Keywords: Hydrogen peroxide
Fenton reaction
Seawater
High performance liquid
chromatograph
Hydroxyl radical
Issue Date: 28-Aug-2008
Publisher: Elsevier
Citation: Olasehinde, E. F., Makino, S., Kondo, H., Takeda, K., & Sakugawa, H. (2008). Application of Fenton reaction for nanomolar determination of hydrogen peroxide in seawater. Analytica Chimica Acta, 627(2), 270–276. doi:10.1016/j.aca.2008.08.026
Abstract: A simple and sensitive method for the determination of nanomolar levels of hydrogen peroxide (H2O2) in seawater has been developed and validated. This method is based on the reduction of H2O2 by ferrous iron in acid solution to yield hydroxyl radical (•OH) which reacts with benzene to produce phenol. Phenol is separated from the reaction mixture by reversed phase high performance liquid chromatography and its fluorescence intensity signals were measured at excitation and emission of 270 and 298 nm, respectively. Under optimumconditions, the calibration curve exhibited linearity in the range of (0–50)×103 nmol L−1 H2O2. The relative standard deviations for five replicate measurements of 500 and 50 nmol L−1 H2O2 are 1.9 and 2.4%, respectively. The detection limit for H2O2, defined as three times the standard deviation of the lowest standard solution (5 nmol L−1 H2O2) in seawater is 4 nmol L−1. Interference of nitrite ion (NO2 −) on the fluorescence intensity of phenol was also investigated. The result indicated that the addition of 10 mol L−1 NO2 − to seawater samples showed no significant interference, although, the addition of 50 mol L−1 NO2 − to the seawater samples decreases the fluorescence intensity signals of phenol by almost 40%. Intercomparison of this method with well-accepted (p-hydroxyphenyl) acetic acid (POHPAA)-FIA method shows excellent agreement. The proposed method has been applied on-board analysis of H2O2 in Seto Inland seawater samples.
Description: Staff Publication
URI: 10.1016/j.aca.2008.08.026
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/815
Appears in Collections:Research Articles

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