Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/844
Title: Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8
Authors: Ademakinwa, Adedeji Nelson
Ayinla, Zainab Adenike
Agboola, Femi Kayode
Keywords: Mutagenesis;
Plackett-Burman design;
DNA fingerprinting RAPDPCR;
Box-Behnken design; RSM
RSM
Issue Date: 4-Jul-2017
Publisher: Elsevier
Citation: Ademakinwa, A.N., Ayinla, Z.A., & Agboola, F. (2017). Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8. Journal of Genetic Engineering & Biotechnology, 15, 345 - 358.
Abstract: Strain improvement of a low fructosyltransferase-producing Aureobasidium pullulans NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide and ethyl methane sulfonate. The wild-type and mutant strain were distinguished using Random amplified polymorphic DNA PCR and DNA fingerprinting analysis. Plackett-Burman and Box Behnken design were statistical tools used to determine important media parameters and optimization, respectively. Phenotypically and genetically, the new improved strain was different from the wild-type. The most important media parameters from PDB influencing fructosyltransferase production were ammonium chloride, sucrose and yeast extract at p<0.05. Some significant parameters obtained with the BBD exhibited quadratic effects on FTase. The F values (35.37 and 32.11), correlation coefficient (0.98 and 0.97) and the percent coefficient of variation (2.53% and 2.40%) were obtained for extracellular and intracellular FTase respectively. The validation of the model in the improved strain resulted in an overall 6.0 and 2.0-fold increase in extracellular and intracellular FTase respectively compared to the wild-type. A relatively low FTase-producing strain of Aureobasidium pullulans NAC8 was enhanced for optimum production using a two-pronged approach involving mutagenesis and statistical optimization. The improved mutant strain also had remarkable biotechnological properties that make it a suitable alternative than the wild-type
Description: Staff Publication
URI: DOI:10.1016/j.jgeb.2017.06.012
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/844
Appears in Collections:Research Articles

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