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Title: Growth and biosurfactant synthesis by Nigerian hydrocarbon-degrading estuarine bacteria
Authors: Adebusoye, Sunday A.
Amund, Olukayode O.
Ilori, Matthew O.
Domeih, Dupe O.
Okpuzor, Joy
Keywords: Corynebacterium sp.
Saccharomyces cerevisae
Flavobacterium sp.
Issue Date: Dec-2008
Publisher: http://creativecommons. org/licenses/by/3.0
Citation: Adebusoye, et al. (2008). Growth and Biosurfactant Synthesis by Nigerian Hydrocarbon-Degrading Estuarine Bacteria. Rev Biol Trop.;56(4):1603-11
Abstract: The ability of microorganisms to degrade petroleum hydrocarbons is important for finding an environmentally-friendly method to restoring contaminated environmental matrices. Screening of hydrocarbon utilizing and biosurfactant-producing abilities of organisms from an estuarine ecosystem in Nigeria, Africa, resulted in the isolation of five microbial strains identified as Corynebacterium sp. DDVl, Flavobacterium sp. DDV2, Micrococcus roseus DDV3,Pseudomonas aeruginosa DDV4 and Saccharomyces cerevisae DDV5. These isolates grew readily on several hydrocarbons including hexadecane, dodecane, crude oil and petroleum fractions. Axenic cultures of the organisms utilized diesel oil (1.0%v/v) with generation times that ranged significantly (t-test, P < 0.05) between 3.25 and 3.88 day, with concomitant production of biosurfactants. Kinetics of growth indicates that biosurfactant synthesis occurred predominantly during exponential growth phase, suggesting that the bioactive molecules are primary metabolites. Strains DDV1 and DDV4 were evidently the most metabolically active in terms of substrate utilization and biosurfactant synthesis compared to other strains with respective emulsification index of 63 and 78%. Preliminary biochemical characterization indicates that the biosurfactants are heteropolymers consisting of lipid, protein and carbohydrate moieties. The hydrocarbon catabolic properties coupled with biosurfactant-producing capacibilities is an asset that could be exploited for cleanup of oil-contaminated matrices and also in food and cosmetic industries.
ISSN: 0034-7744
Appears in Collections:Research Articles

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