The degradation of coniferyl alcohol and the complementary production of chlorogenic acids in the growth culture of Streptomyces albogriseolus KF977548 isolated from decaying wood residues
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Date
2017-01-01
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Coniferyl alcohol is one of the major precursors of lignin; the most abundant aromatic compound and
a natural resource currently receiving attention because of the value-added metabolites resulting from
its degradation. Growth study of Streptomyces albogriseolus KF977548 (strain AOB) isolated from decaying wood residues in a tropical estuarine ecosystem was carried out using coniferyl alcohol as a sole
carbon source. Cell growth and metabolite production were monitored at 24 h interval by dry weight
measurements and HPLC, LC–MS-DAD analyses. Biochemical and PCR assays were carried out to detect
the major catabolic enzymes of interest. Strain AOB utilized coniferyl alcohol completely within 72 h
( = 0.204 h−1, Td = 3.4 h). Laccase and peroxidase were released into the growth medium up to 0.099
and 98 mol/mL respectively. Protocatechuate 3, 4-dioxygenase and demethylase were detected in the
genome whilst ortho-adipate pathway was clearly indicated. Growth on coniferyl alcohol or caffeic acid
as mono substrates resulted in the production of secondary metabolites identified by HPLC–MS as 1-
caffeoylquinic and 3,4,5-tricaffeoylquinic acids, known as chlorogenic acids, in the culture medium. The
microbial production of chlorogenic acids from a lignin-related substrate base by strain AOB could arouse
a plausible biotechnological process.
Description
Keywords
Streptomyces, Chlorogenic acid, Lignin, Enzymes
Citation
Buraimoh, O. M., Ilori, M. O., Amund, O. O., Isanbor, C., & Michel Jr, F. C. (2017). The degradation of coniferyl alcohol and the complementary production of chlorogenic acids in the growth culture of Streptomyces albogriseolus KF977548 isolated from decaying wood residues. Process Biochemistry, 52, 22-29.