BIOCHEMICAL CHANGES IN STREPTOZOTOCIN-INDUCED DIABETIC RATS AFTER TREATMENT WITH ETHANOLIC LEAF EXTRACT OF Croton Zambesicus (Müll. Arg.)
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Date
2016-02-18
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of International Research in Medical and Pharmaceutical Sciences
Abstract
Objective: This study was designed to evaluate the effect of ethanolic leaf extract of C.
zambesicus on total protein (TP), albumin (ALB), globulin (GLO), lactate dehydrogenase (LDH) and
glucose-6-phosphate dehydrogenase (G6PDH) in streptozotocin (STZ) induced diabetic rats.
Methods: Seventy adult male wistar rats were divided into seven groups (n=10). Group A, control
rats; Group B, untreated diabetic rats; Group C, diabetic rats in which C. zambesicus therapy started
2 weeks prior to induction of diabetes; Group D, diabetic rats administered orally with C. zambesicus
leafextract for 2 weeks after the initial four weeks of diabetic induction; Group E, diabetic rats
administered orally with C. zambesicus leaf extract for 4 weeks after the initial four weeks of diabetic
induction; Group F, normal rats administered orally with C. zambesicus leaf extract for four weeks;
Group G, diabetic rats administered with glimepiride (2 mg/kg/day) for four weeks after the
initial four weeks of diabetic induction. At the end of the experimental period, the animals were weighed
and sacrificed. Serum was obtained for TP, ALB, LDH and G6PDH analysis using respective
diagnostic kits.
Results: The results showed an improvement in protein metaboloites (TP, ALB, GLO) whiles the LDH
and G6PDH in the extract and glimepiride treated groups were restored near normal level when
compared with normal control (group A).
Conclusion: In conclusion, this study showed that C. zambesicus leaf extract exerts positive effects
on serum levels of TP, ALB, GLO, LDH and G6PDH in diabetic rats. Thus, ethanolic leaf extract
of Croton zambesicus can be adopted in the management of diabetes mellitus.
Description
Keywords
Albumin, Diabetes, globulin, Glucose-6-phosphate dehydrogenase, Lactate dehydrogenase, Total protein
Citation
OFUSORI, D. A., KOMOLAFE, O. A., ADEWOLE, O. S., ABIODUN, A. A., AYOKA, A. O., & FAKUNLE, J. B. (2016). BIOCHEMICAL CHANGES IN STREPTOZOTOCIN-INDUCED DIABETIC RATS AFTER TREATMENT WITH ETHANOLIC LEAF EXTRACT OF Croton zambesicus (Müll. Arg.). Journal of International Research in Medical and Pharmaceutical Sciences, 18-24.