Amund, Olukayode O.Omole, C. A.Esiobu, N.Ugoji, E. O.2019-06-212019-06-211993Amund, O. O., Omole, C. A., Esiobu, N., & Ugoji, E. O. (1993). Effects of waste engine oil spillage on soil physico-chemical and microbiological properties.http://repository.elizadeuniversity.edu.ng/handle/20.500.12398/113Changes in physic-chemical and microbial properties of soils contaminated with waste motor oil were monitored over a 24 week period. Oil application to soils resulted in a decrease in moisture content but brought about increase in organic matter, total nitrogen and available phosphorus contents. There was an initial decrease in microbial counts followed by a subsequent increase in population levels after four weeks. Microbial species diversity was however reduced in oil-contaminated sites relative to the control sites. Hydrocarbon-utilizing bacteria isolated from the experimental sites were identified as Pseudomonas, Acinetobacter, Alcaligenes, Flavobacterium and Corynebacterium. The organisms grew on long-chain n-alkanes, crude oil and fresh engine oil while a few species grew on aromatic hydrocarbons. Laboratory biodegradation studies of fresh engine oil using strains of Pseudomonas, Acinetobacter, and Corynebacterium showed a progressive decrease in oil concentration and pH of the medium due to the production of acidic metabolites.enOil SpillageBodegradationArticle