Adebusoye, Sunday A.Ilori, Matthew O.Picardal, Flynn W.Amund, Olukayode O.2019-06-202019-06-202008-01-01Adebusoye, et al (2008). Co-metabolic Degradation of Polychlorinated Biphenyls (PCBs) by Axenic Cultures of Ralstonia sp. Strain SA-5 and Pseudomonas sp. Strain SA-6 obtained from Nigerian Contaminated Soils. World J Microbiol Biotechnol 24:61–6810.1007/s11274-007-9438-zhttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/66Substantial metabolism of 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-tetraCB) and 2,3′,4′,5-tetraCB by axenic cultures of Ralstonia sp. SA-5 and Pseudomonas sp. SA-6 was observed in the presence of biphenyl supplementation, although, the strains were unable to utilize tetrachlorobiphenyls as growth substrate. The former was more amenable to aerobic degradation (∼70% degradation) than the latter (22–45% degradation). Recovery of 2,5-chlorobenzoic acid and chloride from 2,3′,4′,5-tetraCB assay is an indication of initial dioxygenase attack on the 3,4-dichlorophenyl ring. The PCB-degradative ability of both strains was also investigated by GC analysis of individual congeners in Aroclor 1242 (100 ppm) following 12-day incubation with washed benzoate-grown cells. Results revealed two different catabolic properties. Whereas strain SA-6 required biphenyl as inducer of the degradation activity, such induction was not required by strain SA-5. Nearly all the detectable congeners in the mixture were extensively degraded (% reduction in ECD area counts for individual congeners ranged from 50.0 to 100% and 14.2 to 100%, respectively, for SA-5 and SA-6). The two strains exhibited no noticeable specificity for congeners with varying numbers of chlorine substitution and positions. The degradative competence of these isolates most especially SA-5 makes them among the most versatile PCB-metabolizing organisms yet reported.enBacteriaPseudomonas spRalstonia sp.BiphenylChlorobenzoic acidArticle