Comparative Evaluation of Lactobacilli and Bifidobacteria in Healthy Individuals
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Date
2020-11-11
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Probiotics & Health
Abstract
Lactobacillus and Bifidobacterium spp. are known as the major group that constitutes probiotic bacteria that form
part of the normal biota of humans. The ecological significance of probiotic bacteria in the human gut cannot
be exaggerated as studies have revealed many of the health benefits they offer. However, much is yet to be known
about its population variation between and within individuals. Five apparently healthy volunteers were recruited
and advised not to consume yogurt, antibiotics, alcohol and steroid three weeks before and during the study. Three
(3) fecal samples from each participant obtained at two weeks interval (total of 15 samples a week) for six weeks
were examined for each subject bacteria. Samples were collected in sterile specimen jars and immediately taken
to the laboratory for analysis. Rogosa and BIM-25 selective media were used for the enumeration of Lactobacillus
spp. and Bifidobacterium spp. respectively using plate count method. Lactobacilli were classified at the specie level
using API 50 CHL kit, while Bifidobacteria isolates were identified at the genus level by detecting the presence
of Fructose-6-phosphate phosphorketolase (F6PPK) activity. Both groups of bacteria were also identified at the
genus level using a genus-specific primer set. All selected isolates obtained from the respective culture media were
confirmed to be Lactobacilli and Bifidobacteria. The data obtained showed that intra-individual variation of the
population of Lactobacillus and Bifidobacterium spp. was significantly lower than inter-individual variation at P<0.05.
This study establishes the fact that the composition level of Lactobacillus and Bifidobacterium spp. varies between
healthy individuals, but with little or no variation within healthy individuals.
Description
Staff Publication
Keywords
Lactobacilli;, Bifidobacteria;, Intra-individual;, Inter-individual;, Variation;, Population;, Gut
Citation
Fadare OS, Sabri S (2020) Comparative Evaluation of Lactobacilli and Bifidobacteria in Healthy Individuals. J Prob Health. 8:224. DOI: 10.35248/2329-8901.20.8:224.