Application of Fenton reaction for nanomolar determination of hydrogen peroxide in seawater
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Date
2008-08-28
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
A simple and sensitive method for the determination of nanomolar levels of hydrogen peroxide
(H2O2) in seawater has been developed and validated. This method is based on the
reduction of H2O2 by ferrous iron in acid solution to yield hydroxyl radical (•OH) which reacts
with benzene to produce phenol. Phenol is separated from the reaction mixture by reversed
phase high performance liquid chromatography and its fluorescence intensity signals were
measured at excitation and emission of 270 and 298 nm, respectively. Under optimumconditions,
the calibration curve exhibited linearity in the range of (0–50)×103 nmol L−1 H2O2. The
relative standard deviations for five replicate measurements of 500 and 50 nmol L−1 H2O2 are
1.9 and 2.4%, respectively. The detection limit for H2O2, defined as three times the standard
deviation of the lowest standard solution (5 nmol L−1 H2O2) in seawater is 4 nmol L−1. Interference
of nitrite ion (NO2
−) on the fluorescence intensity of phenol was also investigated.
The result indicated that the addition of 10 mol L−1 NO2
− to seawater samples showed no
significant interference, although, the addition of 50 mol L−1 NO2
− to the seawater samples
decreases the fluorescence intensity signals of phenol by almost 40%. Intercomparison of
this method with well-accepted (p-hydroxyphenyl) acetic acid (POHPAA)-FIA method shows
excellent agreement. The proposed method has been applied on-board analysis of H2O2 in
Seto Inland seawater samples.
Description
Staff Publication
Keywords
Hydrogen peroxide, Fenton reaction, Seawater, High performance liquid, chromatograph, Hydroxyl radical
Citation
Olasehinde, E. F., Makino, S., Kondo, H., Takeda, K., & Sakugawa, H. (2008). Application of Fenton reaction for nanomolar determination of hydrogen peroxide in seawater. Analytica Chimica Acta, 627(2), 270–276. doi:10.1016/j.aca.2008.08.026