Alterations of Na+/K+-ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats
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Date
2016-07-13
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Background: This study assessed the possible protective mechanisms of protocatechuic acid (PCA) against
cadmium (Cd)-induced oxidative stress and neurotoxicity in rats.
Methods: Male wistar strain rats weighing between 150–160 g were purchased and acclimatized for two
weeks. The rats were divided into seven groups of seven each; NC group received normal saline, CAD
group received 6 mg/kg of Cd-solution, CAD + PSG group received Cd-solution and prostigmine (5 mg/kg),
CAD + PCA-10 and CAD + PCA-20 groups received Cd-solution and PCA (10 mg/kg and 20 mg/kg)
respectively, PCA-10 and PCA-20 groups received 10 mg/kg and 20 mg/kg PCA each. Animals were
administered normal saline, Cd and PCA daily by oral gavage for 21 days. After which the animals were
sacrificed, the brain excised, homogenized and centrifuged. The activities of enzymes (Na+/K+-ATPase,
cholinesterases, catalase, glutathione peroxidase, superoxide dismutase) and levels of oxidative stress
markers (lipid peroxidation and reduced glutathione) linked to neurodegeneration were subsequently
assessed.
Results: Significant (p < 0.05) alterations in the enzyme activities and levels of oxidative stress markers
were observed in CAD group when compared to the NC group. However, the activities of the enzymes
were reversed in CAD + PSG and CAD + PCA groups.
Conclusions: PCA may protect against cadmium-induced neurotoxicity by altering the activities of Na+/K+-
ATPase, acetylcholinesterase, butyrylcholinesterase and endogenous antioxidant enzymes.
Description
Staff Publication
Keywords
Cadmium;, Protocatechuic acid;, Enzymes;, Na+/K+ ATPase;, Cholinesterases;, Antioxidant
Citation
Adefegha, S. A., Oboh, G., Omojokun, O. S., & Adefegha, O. M. (2016). Alterations of Na + /K + -ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomedicine & Pharmacotherapy, 83, 559–568. doi:10.1016/j.biopha.2016.07.017