Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its antiinflammatory and antioxidative activities in human erythrocytes
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Date
2020-06-09
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of
Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using
HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization,
albumin Denaturation and heatinduced hemolysis, malondialdehyde (MDA), and reduced glutathione
(GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human
erythrocytes. The fresh portion (89.12 µg/ml) exhibited the highest potential to inhibit heat-induced
hemolysis compared to the standard drug—Diclofenac (91.51 µg/ml). Freeze-dried sample showed the
highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 µg/ml], [Diclofenac-318.63
µg/ml]) and membrane destabilization ([Freeze-dried-331.93 µg/ml], [Diclofenac-289.57 µg/ml]) when
compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level
and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP)
were treated with the extract. This study confirms the retention of a considerable quantity of bioactive
constituents of plants when freeze-dried.
Description
Keywords
Anti-inflammatory,, Antioxidative, Bryophyllum pinnatum, Drying
Citation
OmojokunOS, Oboh G, Ademiluyi AO, Oladele JO, Boligon AA. Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its antiinflammatory and antioxidative activities in human erythrocytes. J Food Biochem. 2020;00:e13298. https://doi. org/10.1111/jfbc.13298