Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/91
Title: Purification and properties of an α-amylase produced by a cassava-fermenting strain of Micrococcus luteus
Authors: Ilori, Matthew O.
Amund, Olukayode O.
Omidiji, O.
Keywords: Fermentation
Micro organisms
Issue Date: 1-Oct-1997
Publisher: Springer Netherlands
Citation: Ilori, M. O, Amund, O.O. and Omidiji, O. (1997) Purification and Properties of an A-Amylase Produced by a Cassava-Fermenting Strain of Micrococcus Luteus. Folia Microbiologica 42 (5)
Abstract: An extracellular α-amylase produced by a cassava-fermenting strain of Micrococcus luteus was purified 26-fold by gel filtration and ion-exchange chromatography. The molar mass was estimated to be approximately 56 kDa. The optimum temperature of the enzyme was 30oC, optimum pH 6.0 and optimum substrate concentration was 0.6 % (w/v). Treatment of the enzyme at 70 oC for 10 min resulted in 70 % loss of activity. The activation energy was determined to be 34.8 kj/mol. The activity of the enzyme was enhanced by Mg2+, Ca2+, K+, Na+ and inhibited by EDTA, KCN and citric acid. The enzyme may find some application in local food processing.
URI: 10.1007/BF02826551
http://165.22.87.194:8080/jspui/handle/20.500.12398/91
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