Nutritional and Immunomodulatory Activities of Extra Virgin Olive Oil in the Treatment of Diarrhoea in Albino Rats Infected with Escherichia Coli 0157:H7
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Date
2014-10-05
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Publisher
The Journal of Agriculture and Natural Resources Sciences
Abstract
Nutrition refers to the study or intake of food considered in relation to the
body’s dietary needs while immunomodulation refers to changes in the activity
of the immune system caused by factors inhibiting the functions of the immune
system, hence, studies aimed at modifying the immune response to prevent
infections. In this present study, the effectiveness/imunomodulatory effect of
extra virgin olive oil (EOO) supplementation in the diet of experimental
animals (albino rats) infected with E scherichia coli 0157:H7 to cause diarrhoea
was studied. Effects of EOO on weight gain and haematological profile of mice
was also monitored. E. coli counts were performed in both animal stool (feces)
and urine. For the experiment, 16 albino rats were assigned at random to four
(4) groups designated as A, B, C, and D, (four animals per group) and all were
aged between 4-6 weeks. The rats were acclimatized for ten (10) days and
subsequently monitored for seven (7) days (experimental period). The result of
the experiment showed that the infectivity dose of E. coli 0157:H7 in albino
rats was 1.7×103 Cfu/ml. The animals in groups A-C were inoculated orally
(1.7×103Cfu/ml/mouse) while animals in group D were used as control. Loss in
weights occurred in animals when infection set. The E. coli 0157:H7 infection
caused diarrhoea which was treated with extra virgin olive oil (A),
chloramphenicol (B), Not treated at all (C). The treatment with extra virgin
olive oil was however not as effective as the standard antibiotic
(chloramphenicol) in infected animals. The haematological profile of the rats
revealed high Red Blood Cell counts (RBC), Packed Cell Volume (PCV),
Heamoglobin (Hb), low White Blood Cell counts (WBC) and WBC differential
counts revealed higher lymphocyte counts, low neutrophil and monocyte
counts, normal counts for eosinophils and basophils when compared with the
control group (D). Also the only microorganisms isolated from the extra virgin
olive oil (EOO) were identified as Staphylococcus epidermidis. The results
obtained in this study has shown that extra virgin oil has antibacterial property
and that it was able to cure diarrhoea caused by E. coli 0157:H7 in albino rats.
Description
Staff Publication
Keywords
Nutritional,, Immunomodulatory,, Olive Oil,, Albino Rats.
Citation
Oladunmoye, M.K., B.I. Oso2 and A.O. Momoh. 2014. Nutritional and Immunomodulatory Activities of Extra Virgin Olive Oil in the Treatment of Diarrhoea in Albino Rats Infected With Escherichia Coli 0157:H7. The Journal of Agriculture and Natural Resources Sciences. 1(1):45- 56.