Production of cellulase by Mucor ramanniacus using submerged fermentation and its applications in biodegradation of agro-industrial waste
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Date
2021-03-13
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
This study was undertaken to isolate and identify a novel cellulase-producing strain from a waste site
(7°28’11’’N 4°31’24’’E), optimise the growth conditions, partially purify and biochemically characterise the
enzyme. The potentials of the puried cellulase to hydrolyse the lignocellulosic component of some agroindustrial
wastes (e.g. orange peels etc.) was also investigated.
The best cellulase-producing fungus was identied as Mucor ramanniacus and the optimum conditions
for cellulase production were pH (4.5), inoculum size (12 mm), carbon and nitrogen sources were
carboxymethyl cellulose and sodium nitrate respectively resulting in a specic activity of 1423 Units/mg
protein. A purication fold of 1.56 and 45.37 % yield were obtained after purication. The optimum pH
and temperature were at 9.0 and 40°C respectively. The kinetic parameters were 0.63 ± 0.495 mg/ml,
20.21 ± 11.28 U/ml, 1001.4s− 1 for Km and Vmax and kcat respectively. Na+, K+, Ca+, Cysteine, β-
mercaptoethanol and SDS were activators while Tween 80, Triton X-100 EDTA, Hg2+ and Ba2+ inhibited
the enzyme. M. ramanniacus cellulase hydrolysed all agro-industrial wastes used.
The partially puried M. ramanniacus cellulase showed great potential in biodegradation of various
lignocellulosic substrates and the biochemical characteristics exhibited makes it suitable in industrial
applications.
Description
Staff Publication
Keywords
Cellulase,, submerged fermentation,, purification,, Mucor ramanniacus,, agroindustrial wastes