Please use this identifier to cite or link to this item: http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1234
Title: Hydrocarbon Degradation and Biosurfactant Production by an Acenaphthene-degrading Pseudomonas Species
Authors: Salam, Lateef B.
Obayori, Oluwafemi S.
Hawa, Obaje
Keywords: biodegradation;
diesel oil-contaminated soil;
acenaphthene;
crude oil;
biosurfactant;
Pseudomonas
Issue Date: Aug-2016
Publisher: Taylor and Francis: Soil and Sediment Contamination: An International Journal
Citation: Lateef B. Salam, Oluwafemi S. Obayori & Obaje Hawa (2016): Hydrocarbon Degradation and Biosurfactant Production by an Acenaphthene-degrading Pseudomonas Species, Soil and Sediment Contamination: An International Journal, DOI: 10.1080/15320383.2016.1217826
Abstract: An acenaphthene-degrading bacterium putatively identified as Pseudomonas sp. strain KR3 and isolated from diesel-contaminated soil in Lagos, Nigeria was investigated for its degradative and biosurfactant production potentials on crude oil. Physicochemical analysis of the sampling site indicates gross pollution of the soil with high hydrocarbon content (2100 mg/kg) and detection of various heavy metals. The isolate grew luxuriantly on crude oil, engine oil and acenaphthene. It was resistant to septrin, amoxicillin and augmentin but was susceptible to pefloxacin, streptomycin and gentamycin. It was also resistant to elevated concentration of heavy metals such as 1–15 mM lead, nickel and molybdenum. On acenaphthene, the isolate exhibited specific growth rate and doubling time of 0.098 day¡1 and 3.06 days, respectively. It degraded 62.44% (31.2 mg/l) and 91.78% (45.89 mg/l) of 50 mg/l acenaphthene within 12 and 21 days. On crude oil, the specific growth rate and doubling time were 0.375 day¡1 and 1.85 days with corresponding percentage degradation of 33.01% (903.99 mg/l) and 87.79% (2403.71 mg/l) of crude oil (2738.16 mg/l) within 9 and 18 days. Gas chromatographic analysis of residual crude oil at the end of 18 days incubation showed significant reductions in the aliphatic, alicyclic and aromatic fractions with complete disappearance of benzene, propylbenzene, pristane, phytane, and nC18-octadecane fractions of the crude oil. The isolate produced growth-associated biosurfactant on crude oil with the highest emulsification index (E24) value of 72% § 0.23 on Day 10 of incubation. The partially purified biosurfactant showed zero tolerance for salinity and had its optimal activity at 27 C and pH 2.0.
Description: Staff Publication
URI: http://dx.doi.org/10.1080/15320383.2016.1217826
http://repository.elizadeuniversity.edu.ng/jspui/handle/20.500.12398/1234
ISSN: 1549-7887
Appears in Collections:Research Articles

Files in This Item:
File Description SizeFormat 
salam2016.pdf423.06 kBAdobe PDFThumbnail
View/Open


Items in EUSpace are protected by copyright, with all rights reserved, unless otherwise indicated.