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Title: | Co-metabolic Degradation of Polychlorinated Biphenyls (PCBs) by Axenic Cultures of Ralstonia sp. Strain SA-5 and Pseudomonas sp. Strain SA-6 obtained from Nigerian Contaminated Soils |
Authors: | Adebusoye, Sunday A. Ilori, Matthew O. Picardal, Flynn W. Amund, Olukayode O. |
Keywords: | Bacteria Pseudomonas sp Ralstonia sp. Biphenyl Chlorobenzoic acid |
Issue Date: | 1-Jan-2008 |
Publisher: | Springer Netherlands |
Citation: | Adebusoye, et al (2008). Co-metabolic Degradation of Polychlorinated Biphenyls (PCBs) by Axenic Cultures of Ralstonia sp. Strain SA-5 and Pseudomonas sp. Strain SA-6 obtained from Nigerian Contaminated Soils. World J Microbiol Biotechnol 24:61–68 |
Abstract: | Substantial metabolism of 2,3,4,5-tetrachlorobiphenyl (2,3,4,5-tetraCB) and 2,3′,4′,5-tetraCB by axenic cultures of Ralstonia sp. SA-5 and Pseudomonas sp. SA-6 was observed in the presence of biphenyl supplementation, although, the strains were unable to utilize tetrachlorobiphenyls as growth substrate. The former was more amenable to aerobic degradation (∼70% degradation) than the latter (22–45% degradation). Recovery of 2,5-chlorobenzoic acid and chloride from 2,3′,4′,5-tetraCB assay is an indication of initial dioxygenase attack on the 3,4-dichlorophenyl ring. The PCB-degradative ability of both strains was also investigated by GC analysis of individual congeners in Aroclor 1242 (100 ppm) following 12-day incubation with washed benzoate-grown cells. Results revealed two different catabolic properties. Whereas strain SA-6 required biphenyl as inducer of the degradation activity, such induction was not required by strain SA-5. Nearly all the detectable congeners in the mixture were extensively degraded (% reduction in ECD area counts for individual congeners ranged from 50.0 to 100% and 14.2 to 100%, respectively, for SA-5 and SA-6). The two strains exhibited no noticeable specificity for congeners with varying numbers of chlorine substitution and positions. The degradative competence of these isolates most especially SA-5 makes them among the most versatile PCB-metabolizing organisms yet reported. |
URI: | 10.1007/s11274-007-9438-z http://165.22.87.194:8080/jspui/handle/20.500.12398/66 |
Appears in Collections: | Research Articles |
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