Browsing by Author "Adebusoye, S. A."
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Item Biodegradation of p-Chloroaniline by Bacteria Isolated from Contaminated Sites(2010) Fashola, M. O.; Obayori, O. S.; Omotayo, A. E.; Adebusoye, S. A.; Amund, Olukayode O.Enrichment of water from a contaminated site in a textile industry in Ikeja resulted in the isolation of two bacteria belonging to the genera Alcaligenes and Cellulomonas. These bacteria were able to mineralize para-chloroaniline (p-chloroaniline). Time course degradation of p-chloroaniline using pure cultures of these organisms showed that p-chloroaniline supported the growth of these isolates. An initial increase in cell densities of 7.50-9.46 cfu/mL was recorded from day 0-9th day for Cellulomonas sp. while for Alcaligenes denitrificans it was 7.20-9.40 cfu/mL. After day 9, a decrease in population occurred, indicating non-availability of nutrients or toxicity of the medium. Simultaneously, a decrease in the pH, indicative of increased acidity of the medium, was also observed from the first day. The result of the GC analysis of the pure isolates on p-chloroaniline shows that 86.5% of the p-chloroaniline was degraded by the Cellulomonas sp. while 81.2% was degraded by the A. denitrificans in 30 days. These bacterial isolates utilized other hydrocarbons such as pyrene, anthracene, crude oil and chlorobenzoates as sole source of carbon and energy but not phenanthrene, naphthalene and biphenyl. The two isolates tolerated NaCl concentration of up to 5%.Item A Comparative Study of Biosurfactant Synthesis by Pseudomonas aeruginosa Isolated from Clinical and Environmental Samples(Taylor & Francis Group, 2012-02-15) Agwu, O. A.; Ilori, Matthew O.; Adebusoye, S. A.; Amund, Olukayode O.Evaluation of emulsifying activities indicates that biosurfactants were produced by an environmental (strain EP1) and a clinical (strain CP1) species of Pseudomonas aeruginosa. During growth on hydrocarbons, the organisms produced biosurfactants. Both strains grew luxuriantly on motor oil and readily synthesized abundant surfactants at the expense of easily metabolizable substrates. During a 12-day cultivation on motor oil, the organisms produced growth-associated extracellular surfactants with emulsification activities of 71 and 38% for EP1 and CP1, respectively. The generation times obtained for EP1 and CP1 were 1.74 and 2.66 days. The biosurfactants that could not be secreted on glucose were partially purified and putatively identified as rhamnolipids. The surface-active compounds present high emulsification activity and stability in the pH range of 3.0–10.0, temperature range of 4°C–100°C, and salinity range of 16–44% and are capable of stabilizing oil-in-water emulsions with several hydrocarbons. Typical emulsions produced were stable for several weeks. The results also showed that the biosurfactants were able to remove a significant amount of crude oil from contaminated soil; for instance, strain EP1 surfactant removed 54%, CP1 41%, detergent 42%, and water 30%. The rhamnolipids from these strains represent a new class of biosurfactants that have potential for use in a variety of biotechnological and industrial applications where extremes of pH, thermal, and saline conditions would have little or no effect on activity.