Browsing by Author "Ademiluyi, Adedayo O."

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    Drying methods alter angiotensin-I converting enzyme inhibitory activity, antioxidant properties, and phenolic constituents of African Mistletoe (Loranthus bengwensis L) Leaves
    (SAGE Publications, 2016-10) Oboh, Ganiyu; Omojokun, Olasunkanmi S.; Ademiluyi, Adedayo O.
    This study investigated the most appropriate drying method (sun drying, oven drying, or air drying) for mistletoe leaves obtained from almond tree. The phenolic constituents were characterized using high-performance liquid chromatography–diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on angiotensin-I converting enzyme (ACE) was determined in vitro as also the antioxidant properties. Oven-dried extract (kidney [276.09 μg/mL] and lungs [303.41 μg/mL]) had the highest inhibitory effect on ACE, while air-dried mistletoe extract (kidney [304.47 μg/mL] and lungs [438.72 μg/mL]) had the least. Furthermore, the extracts dose-dependently inhibited Fe2+ and sodium nitroprusside-induced lipid peroxidation in rat’s heart and kidney. Also, all extracts exhibited antioxidative properties as typified by their radical scavenging and Fe-chelating ability. Findings from this study revealed that oven drying is the best of the 3 drying methods used for mistletoe obtained from almond host tree, thus confirming that diversity in drying methods leads to variation in phenolic constituents and biological activity of plants.
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    Effects of drying on cholinesterases and angiotensin-I converting enzyme inhibitory potential and phenolic constituents of African mistletoe (Loranthus bengwensis L) leaves from kolanut host tree
    (Wiley, 2017-12-15) Omojokun, Olasunkanmi S.; Oboh, Ganiyu; Ademiluyi, Adedayo O.
    This study investigated the most appropriate drying method (sun drying, oven drying, and shade drying) for mistletoe leaves obtained from kolanut tree. The phenolic constituents were characterized using high performance liquid chromatography–diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on cholinesterases and angiotensin-I converting enzyme (ACE) and antioxidant activities were determined in vitro. The extracts inhibited acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ACE in dose-dependent manner. However, extract from sun-dried sample exhibited the highest AChE, BChE, and ACE inhibitory effect while extract from shade-dried sample had the least. Likewise, sun-dried sample exhibited the highest antioxidant properties as exemplified by Fe21-chelating, 1,1-diphenyl-2 picrylhdrazyl, OH, and nitric oxide radical scavenging abilities. This study also revealed the presence of 20 phenolic compounds with caffeic acid being the most predominant. Conclusively, kolanut host tree mistletoe leaves can be used as therapeutic agent in the management of Alzheimer’s disease and hypertension.
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    Effects of drying on cholinesterases and angiotensin‐I converting enzyme inhibitory potential and phenolic constituents of African mistletoe (Loranthus bengwensis L) leaves from kolanut host tree
    (Wiley Online Library, 2018-08) Omojokun, Olasunkanmi S.; Oboh, Ganiyu; Ademiluyi, Adedayo O.
    This study investigated the most appropriate drying method (sun drying, oven drying, and shade drying) for mistletoe leaves obtained from kolanut tree. The phenolic constituents were characterized using high performance liquid chromatography–diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on cholinesterases and angiotensin‐I converting enzyme (ACE) and antioxidant activities were determined in vitro. The extracts inhibited acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ACE in dose‐dependent manner. However, extract from sun‐dried sample exhibited the highest AChE, BChE, and ACE inhibitory effect while extract from shade‐dried sample had the least. Likewise, sun‐dried sample exhibited the highest antioxidant properties as exemplified by Fe2+‐chelating, 1,1‐diphenyl‐2 picrylhdrazyl, OH, and nitric oxide radical scavenging abilities. This study also revealed the presence of 20 phenolic compounds with caffeic acid being the most predominant. Conclusively, kolanut host tree mistletoe leaves can be used as therapeutic agent in the management of Alzheimer's disease and hypertension.
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    Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its anti-inflammatory and antioxidative activities in human erythrocytes
    (Wiley, 2020-05-05) Omojokun, Olasunkanmi S.; Oboh, Ganiyu; Ademiluyi, Adedayo O.; Oladele, Josephine O.; Boligon, Aline A.
    The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization, albumin Denaturation and heatinduced hemolysis, malondialdehyde (MDA), and reduced glutathione (GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human erythrocytes. The fresh portion (89.12 μg/ml) exhibited the highest potential to inhibit heat-induced hemolysis compared to the standard drug—Diclofenac (91.51 μg/ml). Freeze-dried sample showed the highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 μg/ml], [Diclofenac-318.63 μg/ml]) and membrane destabilization ([Freeze-dried-331.93 μg/ml], [Diclofenac-289.57 μg/ml]) when compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP) were treated with the extract. This study confirms the retention of a considerable quantity of bioactive constituents of plants when freeze-dried. Practical applications The ideal method of drying Bryophyllum pinnatum and possible anti-inflammatory potential was investigated. This work may apply to the development of anti-inflammatory agents from a natural source with little or no side effect in managing inflammation.
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    Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its antiinflammatory and antioxidative activities in human erythrocytes
    (Wiley, 2020-06-09) Omojokun, Olasunkanmi S.; Oboh, Ganiyu; Ademiluyi, Adedayo O.; Oladele, Josephine O.; Boligon, Aline A.
    The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization, albumin Denaturation and heatinduced hemolysis, malondialdehyde (MDA), and reduced glutathione (GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human erythrocytes. The fresh portion (89.12 µg/ml) exhibited the highest potential to inhibit heat-induced hemolysis compared to the standard drug—Diclofenac (91.51 µg/ml). Freeze-dried sample showed the highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 µg/ml], [Diclofenac-318.63 µg/ml]) and membrane destabilization ([Freeze-dried-331.93 µg/ml], [Diclofenac-289.57 µg/ml]) when compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP) were treated with the extract. This study confirms the retention of a considerable quantity of bioactive constituents of plants when freeze-dried.
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    In Vitro Studies on the Antioxidant Property and Inhibition of 𝛼-Amylase, 𝛼-Glucosidase, and Angiotensin I-Converting Enzyme by Polyphenol-Rich Extracts from Cocoa (Theobroma cacao) Bean
    (Hindawi, 2014) Oboh, Ganiyu; Ademosun, Ayokunle O.; Ademiluyi, Adedayo O.; Omojokun, Olasunkanmi S.; Nwanna, Esther E.; Longe, Kuburat O.
    This study sought to investigate the antidiabetic and antihypertensive mechanisms of cocoa (Theobroma cacao) bean through inhibition of 𝛼-amylase, 𝛼-glucosidase, angiotensin-1 converting enzyme, and oxidative stress. Methodology. The total phenol and flavonoid contents of the water extractable phytochemicals from the powdered cocoa bean were determined and the effects of the extract on 𝛼-amylase, 𝛼-glucosidase, and angiotensin-1 converting enzyme activities were investigated in vitro. Furthermore, the radicals [1,1-diphenyl-2 picrylhydrazyl (DPPH), 2,2..-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), hydroxyl (OH), and nitric oxide (NO)] scavenging ability and ferric reducing antioxidant property of the extract were assessed. Results. The results revealed that the extract inhibited 𝛼-amylase (1.81 ± 0.22 mg/mL), 𝛼-glucosidase (1.84 ± 0.17 mg/mL), and angiotensin-1 converting enzyme (0.674 ± 0.06 mg/mL [lungs], 1.006 ± 0.08 mg/mL [heart]) activities in a dose-dependent manner and also showed dose-dependent radicals [DPPH (16.94 ± 1.34 mg/mL), NO (6.98 ± 0.886 mg/mL), OH (3.72 ± 0.26 mg/mL), and ABTS (15.7 ± 1.06 mmol/TEAC⋅g] scavenging ability. Conclusion. The inhibition of 𝛼-amylase, 𝛼-glucosidase, and angiotensin-1 converting enzyme activities by the cocoa bean extract could be part of the possible mechanism by which the extract could manage and/or prevent type-2 diabetes and hypertension.