Browsing by Author "Amund, O.O."

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    FACTORS THAT INFLUENCE METHANOGENIC ACTIVITIES IN A LOW SULFATE OIL-PRODUCING FACILITY
    (Taylor and Francis Online, 2018-04-26) Okoro, C.C. ;; Nwezza, E.E ;; Amund, O.O.
    Molecular analysis of a low sulfate oil bearing environment was carried out using 16S rRNA gene sequencing technique and the effects of some environmental factors like pH, temperature, salinity on methane production and corrosion rates were determined. Pyrosequenced data revealed dominance of hydrogenotrophic (Methanobacterium) andMethylotrophic (Methanolobus) in samples. Results showed that samples had their optimal temperature for maximum methane production at 35–40oC and higher temperature (55oC) was inhibitory to methane production. Methanogens in samples produced methane over a wide pH range but the pH optima for maximum methane production ranged between 6 and 7. Sodium chloride was tolerated better at lower concentrations (<200mM), higher concentrations (>300mM) drastically inhibitedmethane formation, an indication that themethanogens involved are not halophytes. Because different methanogens require different substrates and reacts to changes in environmental conditions, environmental factorsmay indirectly control the diversity and activity of methanogens in low sulfate oil bearing environments.
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    In vitro Degradation of Extracted Cassava Linamarin by Bacillus Species Isolated from cassava wastewater
    (Egyptian Academic Journal of Biological Sciences, 2017-07-01) Ogunyemi, A.K. ;; Samuel, T.A. ;; Buraimoh, O.M. ;; Amund, O.O.; Ilori, M. O.;; Alagbada, B. C.;; Olumuyiwa, E.O.;; Odetunde, S. K.;; Akinrodoye, F.
    Linamarin is the most abundant cyanoglucoside present in cassava cells and may generate the equivalent amount of hydrocyanic acid. This study was aimed to assess degradative capacities of Bacillus pumilus strain WOB3 and WOB7on linamarin. The test organisms for linamarase activity were identified on the basis of phenotype, biochemical properties and 16S rDNA gene sequencing as: Bacillus pumilus strain WOB3 KX774195 and Bacillus pumilus strain WOB7 KX774196. Growth studies showed that the strains grew in all the substrates tested. The doubling times of Bacillus pumilus strain WOB3 and Bacillus pumilus strain WOB7 were 8.25 d and 7.53 d on cassava effluent, 6.30 dand 5.78 d on supplemented cassava effluent, 8.66 dand 9.90 d on waste leachate and 6.30 d and 9.24 d on supplemented waste leachate respectively; with specific growth rates of 0.084 d-1 and 0.092 d-1 on cassava effluent, 0.11 d-1 and 0.12 d-1 on supplemented cassava effluent, 0.080 d-1 and 0.070 d-1 on waste leachate and 0.11 d-1 and 0.075 d-1 on supplemented waste leachate respectively. High-performance liquid chromatographic analysis studies revealed that linamarin degradation by the strains followed a linamarase pathway involving CO2 and HCN as metabolic intermediates. Based on HPLC analysis, linamarin residual concentration at day 12 by the strains WOB3 and WOB7 was 26.73 mgL-1(19.79 %) and 29.79 mgL-1 (21.92 %). These novel features make the bacteria suitable candidates for in-situ application on sites contaminated with cassava processing wastes.
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    STUDIES ON THE LIFE CYCLE AND MORPHOMETRICS OF HONEYBEES, APIS MELLIFERA ADANSONII (HYMENOPTERA:APIIDAE) IN A MANGROVE AREA OF LAGOS NIGERIA.
    (A Journal of the Faculty of Science Obafemi Awolowo University, Ile-Ife, Nigeria, 2011-11-18) Fasasi, K.A ;; Malaka, S.L.O ;; Amund, O.O.
    The life cycle of the honeybee, Apis mellifera Adansonii, was studied in mangrove area by monitoring the developmental stages and morphology of the castes. It was observed that the fate of the eggs were predetermined at the onset leading to drones, queens or workers. It was also established that the three different castes (drone, queen and worker) exhibit similar patterns of complete metamorphoses. The mean developmental period of the drone from egg to adult stage was the longest (24.97+1.77 days, n=60) followed by that of worker (20.22+2.20 days, n=60) and the queen (15.5 + 2.08 days, n=60) in that order. The body length and head capsule width varied within the different castes. The general pattern of the studied life cycle of A. mellifera Adansonii in mangrove area of Lagos is similar to A. mellifera scutellata and Africanized A. mellifera studied in other regions. It was concluded that the knowledge of the life cycle of A. mellifera Adansonii by bee farmers in their locality may enhance their apicultural practices for better productivity.