Browsing by Author "Bello, Temitope Kudirat"

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    Antibiogram of bacterial flora of public health significance associated with postharvest Irvingia gabonensis seeds in Lagos State, Nigeria
    (The Proceedings of the Nigerian Academy of Science, 2020-08-02) Bello, Olorunjuwon Omolaja; Bello, Temitope Kudirat; Babalola, Olubukola Oluranti; Olu-Aderoumu, William Olusola
    The spread of resistant bacteria within the community has continually posed obvious additional problems for infectious control. Efforts to identifying sources of resistant bacteria have not been channeled towards medicinal food condiments. This study investigated the antibiogram of bacterial flora of public health significance associated with postharvest Irvingia gabonensis seeds in Lagos State, Nigeria. The blended, homogenized and serially diluted samples of I. gabonensis seeds were plated using the spread plate technique on selective and differential agar media. API 20E and API 20NE were used for identification of members of Enterobacteriaceae and non-Enterobacteriaceae, respectively. The agar disc-diffusion method was employed to determine the antibiotic resistance profiles of the isolates. A total of 263 bacterial isolates (129 Gram-positive and 134 Gram-negative) were encountered. Eighty-five (66%) of Gram-positive isolates exhibited resistance to penicillin, gentamicin 65 (50.4%), erythromycin 69 (53.5%), cloxacillin 63 (43.8%), chloramphenicol 73 (56.6%), amoxicillin 75 (58.1%), tetracycline 58 (45%) while 69 (53.5%) showed resistance to streptomycin. However, 87 (65%) of Gram-negative bacterial strains exhibited resistance to cloxacillin, ceftazidime 82 (61.2%), ciprofloxacin 67 (50%), gentamicin 77 (57.5%), cefotaxime 74 (55.2%), augmentin 84 (62.7%), nitrofuratoin 61 (45.5%) and 24 (17.9%) to ofloxacin. This study showed that I. gabonensis seeds could be a source of antibiotic-resistant bacterial strains, despite its enormous medicinal properties, which is a threat to public health. The antibiotic resistance patterns of isolated bacterial strains are of medical importance as there are chances of transferring resistant traits.
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    Microbial quality of utility water at universities in Nigeria
    (Malaysian Society for Microbiology, 2020-04-28) Bello, Olorunjuwon Omolaja; Oni, Mathew Olujenyo; Bello, Temitope Kudirat; Fashola, Muibat Omotola; Oluwafemi, Yinka Doris
    Aims: Water is described as safe and wholesome when it is free from pathogenic microorganisms and chemical substances that are hazardous to human health. This study aimed to investigate the microbial quality of water used for drinking, cooking, bathing and other purposes at universities in Nigeria. Methodology and results: Water samples were collected from forty-four storage tanks across four selected universities. Total viable bacteria in the water samples were cultivated using the plate count agar. The isolation of total coliform and Escherichia coli were carried out on Harlequin™ E. coli/coliform agar (HA) medium, while media-faecal coliform was used for faecal coliform employing the membrane filtration technique. Physicochemical parameters such as alkalinity, pH, total alkalinity, total dissolved solid, total suspended solid, electrical conductivity, total hardness, fluoride and chloride ion concentrations, were evaluated in accordance with standard procedures. Data were compared statistically using MedCalc statistical software. Considering the heterotrophic bacterial counts, all water samples were unsatisfactory. For the total coliform counts, 50% of samples were satisfactory but suspicious, while remaining 50% were unsatisfactory. Faecal coliforms results showed that 50% of samples gave excellent quality, 25% showed satisfactory but suspicious quality, while 25% showed unsatisfactory result. There were no significant differences in the total viable, total coliform and E. coli counts of water sampled from universities A and D (p>0.05). The predominant bacterial species was Pseudomonas aeruginosa (23.17%), while the least encountered was Salmonella typhimurium (2.44%). All physicochemical parameters tested were within the acceptable limit. Conclusion, significance and impact of study: This study revealed that the water used by students of studied universities was contaminated with potential bacterial pathogens. However, all physicochemical parameters tested were within the permissible standard limits and satisfied the requirements for domestic utility.