Browsing by Author "Boligon, Aline A."

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    Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its anti-inflammatory and antioxidative activities in human erythrocytes
    (Wiley, 2020-05-05) Omojokun, Olasunkanmi S.; Oboh, Ganiyu; Ademiluyi, Adedayo O.; Oladele, Josephine O.; Boligon, Aline A.
    The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization, albumin Denaturation and heatinduced hemolysis, malondialdehyde (MDA), and reduced glutathione (GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human erythrocytes. The fresh portion (89.12 μg/ml) exhibited the highest potential to inhibit heat-induced hemolysis compared to the standard drug—Diclofenac (91.51 μg/ml). Freeze-dried sample showed the highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 μg/ml], [Diclofenac-318.63 μg/ml]) and membrane destabilization ([Freeze-dried-331.93 μg/ml], [Diclofenac-289.57 μg/ml]) when compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP) were treated with the extract. This study confirms the retention of a considerable quantity of bioactive constituents of plants when freeze-dried. Practical applications The ideal method of drying Bryophyllum pinnatum and possible anti-inflammatory potential was investigated. This work may apply to the development of anti-inflammatory agents from a natural source with little or no side effect in managing inflammation.
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    Impact of drying processes on Bryophyllum pinnatum phenolic constituents and its antiinflammatory and antioxidative activities in human erythrocytes
    (Wiley, 2020-06-09) Omojokun, Olasunkanmi S.; Oboh, Ganiyu; Ademiluyi, Adedayo O.; Oladele, Josephine O.; Boligon, Aline A.
    The effect of drying on the phytoconstituents, antioxidative, and anti-inflammatory properties of Bryophyllum pinnatum leaves was investigated. The phenolic constituents were characterized using HPLC-DAD. The aqueous extraction was done and various assays (Inhibition of membrane stabilization, albumin Denaturation and heatinduced hemolysis, malondialdehyde (MDA), and reduced glutathione (GSH) contents, as well as superoxide dismutase (SOD) activity), were carried out on human erythrocytes. The fresh portion (89.12 µg/ml) exhibited the highest potential to inhibit heat-induced hemolysis compared to the standard drug—Diclofenac (91.51 µg/ml). Freeze-dried sample showed the highest inhibitory potential on albumin denaturation ([Freeze-dried-330.72 µg/ml], [Diclofenac-318.63 µg/ml]) and membrane destabilization ([Freeze-dried-331.93 µg/ml], [Diclofenac-289.57 µg/ml]) when compared with Diclofenac. Similarly, the freeze-dried sample showed the highest GSH and SOD level and lowest MDA level when human erythrocytes challenged with tertiary butyl hydroperoxide (tBHP) were treated with the extract. This study confirms the retention of a considerable quantity of bioactive constituents of plants when freeze-dried.
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    Starch composition, glycemic indices, phenolic constituents, and antioxidative and antidiabetic properties of some common tropical fruits
    (No longer published by Elsevier, 2016-06-01) Oboh, Ganiyu; Ademosun, Ayokunle O.; Akinleye, Margareth; Omojokun, Olasunkanmi S.; Boligon, Aline A.; Athayde, Margareth L.
    Background Studies have supported the protective effect of high fruit consumption in the management of chronic diseases such as diabetes. Methods Thirteen fresh tropical fruits were sourced for and the fruits juices were extracted, freeze dried, and then reconstituted for analysis. The sugar, starch, amylose, and amylopectin contents as well as glycemic indices, antioxidant properties, and the ability of the fruits to inhibit starch-hydrolyzing enzymes were determined. Also, the phenolic constituents of the fruits were characterized using high-performance liquid chromatography coupled with diode array detector. Results The starch, sugar, amylase, and amylopectin contents were 3.01–3.89 g/100 g, 35.34–60.91 g/100 g, 0.84–1.46 g/100 g, and 1.68–2.86 g/100 g, respectively, while the glycemic indices were 28.01–68.34, with African star apple (28.01) having the lowest and watermelon (68.34) the highest. Furthermore, the fruits exhibited high antioxidant properties as exemplified by their DPPH, ABTS+, OH, and NO radical scavenging abilities. Likewise, the fruits also demonstrated α-amylase and α-glucosidase inhibitory property with Soursop (IC50 = 18.52 μg/mL), guava (IC50 = 19.77 μg/mL), and African star apple (IC50 = 20.86 μg/mL) showing the highest inhibitory potential among the 13 fruits. Similarly, the same trend was followed for α-glucosidase inhibitory activity. Conclusion The fruits' low glycemic indices, strong antioxidant properties, and inhibition of α-amylase and α-glucosidase activities could be possible mechanisms for their use in the management and prevention of type-2 diabetes.