Browsing by Author "Ilori, M. O."
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Item Analysis of Decomposing Wood Wastes in the Lagos Lagoon using Terminal-Restriction Fragment Length Polymorphism and Illumina Sequencing Platform(NIGERIAN JOURNAL OF TECHNOLOGICAL DEVELOPMENT, 2022-06) Buraimoh, O. M.; Adewumi, G. A.; Akinyemi, N. M.; Amund, O. O.; Ilori, M. O.; Michel Jr., F. C.Indiscriminate disposal of wood shavings and sawdust into the Lagos lagoon usually constitute environmental hazard and varying degree of threats to marine biotic communities. In this study we applied terminalrestriction fragment length polymorphism (T-RFLP) and illumina sequencing of 16S ribosomal RNA gene, to describe the microbial ecology of decomposing wood wastes in the Lagos lagoon, Nigeria. The terminal restriction fragments (TRFs) generated corresponds to over 100 bacterial genera and numerous uncultured bacterial clones. Phylogenetic analysis of the TRFs of 16S rRNA gene sequences and cultured bacterial sequences established genetic relatedness and divergent relationships. T-RFLP in combination with illumina sequencing platform identified bacterial species (Acinetobacter sp., Clostridium sp., Planctomyces sp., Escherichia coli, Pantoea agglomerans, Dyella japonica, Ochrobactrum sp., Prevotella sp., Runella slithyformis, Enterobacter aerogenes, Gordonia amicalis, Klebsiella sp., Thalassospira sp. and Serratia sp.) not previously accounted for in culture-dependent analysis. Bacterial strains such as Ruminococcus flavefaciens, Fibrobacter succinogenes, Simiduia agarivorans, Microbulbifer sp. and Cellulomonas fimi further identified in illumina sequences were found to be involved in the biodegradation of lignin and lignin derivatives. Hence, understanding of the diverse autochthonous microbiota of decomposing wood wastes in the Lagos lagoon is essential in the development of effective biotechnology programme, to tackling the menace of sawdust pollution.Item Genotoxicity Evaluation of Landfill Solid Waste Leachates from a Waste Dumpsite in Lagos State, Nigeria using the Allium cepa Assay(Plant and Animal Research Journal, 2018) Ogunyemi, Kayode A.; Samuel, Titilola A.; Amund, Olukayode O.; Ilori, M. O.The management of municipal landfill’s leachate is considered one of the most significant environmental issues. Potential mutagenic and genotoxic effects of solid waste leachates (SWL) were investigated using the Allium cepa root meristematic cells assay technique. In Allium root growth inhibition test, experimental onion bulbs were cultivated in various concentrations of the SWL and distilled water was used as a negative control. The root tips from the treated bulb were processedby orcein squash technique after 72 h. The mean lengths of root bundles were obtained andeffective concentration (EC) values calculated. Some of the physicochemical properties of the WL were also determined. The A. cepa assay was carried out at concentrations of 0.05, 0.1, 2.0, 5, 10, 20, 50 and 100% of the WL. There was a statistically different (p < 0.05), concentrationdependent inhibition of onion root growth and mitotic index, and induction of chromosomal aberrations in the onion test. The results of the physicochemical analysis revealed that the concentrations of some parameters were above the maximum permissible limit set by the world health organization (WHO) and could partly be correlated with the toxicity of wastewater. The landfill leachates pose a risk to human health and the environment in general. Hence, ecotoxicity/genotoxicity assays would be useful in leachate risk assessment when coupled with physicochemical analysis.