Browsing by Author "Ogunyemi, A. K."
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Item Efficacy of intervention strategies for bioremediation of crude oil in polluted soil microcosm(Faculty of Science, Obafemi Awolowo University, 2017) Buraimoh, Olanike M.; Ogunyemi, A. K.; Ibrahim, N. H.; Adebusoye, A. S.; Ilori, Matthew O.; Amund, Olukayode O.Crude oil, though not manmade but largely manipulated by man to provide different oil-based products has become a major source of environmental pollution. This menace on land do contribute to the retardation of vegetation growth and human health hazards, while in water it may be toxic to aquatic animals. The search for the solution to ameliorate the seemingly unending pollution and its side effects necessitated the evaluation on the effect of bioaugmentation, biostimulation and natural attenuation of crude oil pollution in soil microcosms. The bacterial species selected for this study (Bacillus thuringensis strain LG32 and Burkolderia pseudomallei strain A81) were preliminarily identified using the conventional biochemical tests and further identification was carried out using the API kit. The results of the study carried out over a period of five weeks indicated that there was a marked reduction in the available phosphorous and potassium in the bioaugmented and biostimulated soils compared with that of the control. The mean values for total viable counts (TVC) of population of hydrocarbon utilizing bacteria (HUB) was higher in the bioaugmented soil ranged (LG32=6.0-7.5log10cfu g-1 ; A81=5.5- 7.5log10cfug-1 ; LG32+A81=6.0-7.5log10cfug-1 ) compared with that of the control (6.0-6.2log10cfug-1 ). When bioaugmentation was combined with biostimulation, the soil had higher counts of HUB (6.0-9.0log10cfug-1 ) and HUF (3.5-6.5log10cfug-1) compared to bioaugmentation without stimulation (HUB: 6.0-7.5; HUF: 3.5-5.5). The GC result indicated that by day 35, 96.92% of the aliphatic and aromatic components have been degraded in the augmented soil, higher than the natural attenuation control.Item Toxicity evaluation of waste effluent from cassava-processing factory in lagos state, nigeria using the Allium cepa assay(Faculty of Science, Obafemi Awolowo University, 2018) Ogunyemi, A. K.; Samuel, T. A.; Amund, Olukayode O.; Ilori, Matthew O.Mutagenic and genotoxic effects of cassava wastewater (CWW) were investigated by assay of Allium cepa root meristematic cells. The physicochemical parameters of the wastewater samples showing cyanide content were also determined. In Allium root growth inhibition test, experimental onion bulbs were cultivated in various concentrations of the CWW and distilled water was used as a negative control. After 72 h, the root tips from the treated bulb were processed for cytological studies by orcein squash technique. The mean lengths of root bundles were obtained and effective concentration (EC) values calculated. The cytotoxic effects on the onion root tips showed strong growth retardation at high concentrations of the effluent with EC value of 10%. The 50 mitotic index (MI) rapidly decreased with increasing effluent concentration compared to control. There was significant increase in frequency of chromosome aberrations (sticky chromosome, c-mitosis, vagrant chromosome, bridges fragment, binucleated cells, multipolar anaphase, attached chromosome and laggard chromosome) in root tip meristem cells of Allium cepa at all tested concentrations. Further analysis using oneway ANOVA revealed that there was a statistically significant difference (p<0.05) in concentration-dependent inhibition of onion root growth, mitotic index and induction of chromosomal aberration in the Allium cepa test. The results indicate that the effluent samples collected were highly mutagenic. The results of physicochemical analysis revealed that the concentrations of some parameters (conductivity, total suspended solid (TSS), total dissolved solid (TDS), biological oxygen demand (BOD), nitrate, cyanide, chloride and metals-magnesium, aluminum, chromium, cadmium, manganese and iron) were above the maximum permissible limit set by world health organization (WHO) and could partly be correlated with the toxicity of wastewater. The findings indicate that the substances contained in the cassava effluents may be toxic to living organisms and may pollute the environment if untreated.