Browsing by Author "Omotayo, A. E."
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Item Biodegradation of p-Chloroaniline by Bacteria Isolated from Contaminated Sites(2010) Fashola, M. O.; Obayori, O. S.; Omotayo, A. E.; Adebusoye, S. A.; Amund, Olukayode O.Enrichment of water from a contaminated site in a textile industry in Ikeja resulted in the isolation of two bacteria belonging to the genera Alcaligenes and Cellulomonas. These bacteria were able to mineralize para-chloroaniline (p-chloroaniline). Time course degradation of p-chloroaniline using pure cultures of these organisms showed that p-chloroaniline supported the growth of these isolates. An initial increase in cell densities of 7.50-9.46 cfu/mL was recorded from day 0-9th day for Cellulomonas sp. while for Alcaligenes denitrificans it was 7.20-9.40 cfu/mL. After day 9, a decrease in population occurred, indicating non-availability of nutrients or toxicity of the medium. Simultaneously, a decrease in the pH, indicative of increased acidity of the medium, was also observed from the first day. The result of the GC analysis of the pure isolates on p-chloroaniline shows that 86.5% of the p-chloroaniline was degraded by the Cellulomonas sp. while 81.2% was degraded by the A. denitrificans in 30 days. These bacterial isolates utilized other hydrocarbons such as pyrene, anthracene, crude oil and chlorobenzoates as sole source of carbon and energy but not phenanthrene, naphthalene and biphenyl. The two isolates tolerated NaCl concentration of up to 5%.Item Crude Oil Degradation by Microorganisms in Soil Composts.(Academic Journals Inc, 2012) Omotayo, A. E.; Ojo, O. Y.; Amund, Olukayode O.The hydrocarbon utilizing capabilities of Micrococcus varians, Bacillus badius, Corynebacterium ulcerans and Corynebacterium amycolatum isolated from soil composts were analysed. In the biodegradation experiment, utilization of the substrate by bacterial isolates resulted in increased population densities with simultaneous increase in emulsification index (EI 24) valued from 30.0 to 48.84 and a decrease in residual crude oil concentration. The growth rate constants (u) and mean generation (h) times of Micrococcus varians, Bacillus badius, Corynebacterium ulcerans and Corynebacterium amycolatum were 0.027 and 25.5, 0.025 and 27.5, 0.019 and 36.2, 0.023 and 30.0, respectively. The gas chromatographic analysis of the residual hydrocarbons in the liquid medium at the end of 30 days incubation showed that Micrococcus varians had the highest degradation rate of 93.10%, whilst Bacillus badius, Corynebacterium ulcerans and Corynebacterium amycolatum were 0.027 and 25.5, 0.025 and 27.5, 0.019 and 36.2, 0.023 and 30.0, respectively. The gas chromatographic analysis of the residual hydrocarbons in the liquid medium at the end of 30 days incubation showed that Micrococcus varians had the highest degradation rate of 93.10%, whilst Bacillus badius, Corynebacterium ulcerans and Corynebacterium amycolatum had biodegradation rates of 89.22, 88.22 and 90.82%, respectively. The residual hydrocarbon in soil microcosm after 30 days of biodegradation was 73.75, 75.81 and 76.94% for Micrococcus varians, Corynebacterium amycolatum and a consortium of the two isolates, respectively. A substrate specificity test of the isolates on different hydrocarbons showed that the isolates had good growth on anthracene and engine oil but poor growth on pyrene, toluene, naphthalene, dodecane and xylene.Item Microbial Qualities of Vegetables, Water and Soils from Vegetable Gardens in Lagos State, Nigeria(Nigerian Journal of Microbiology, 2017) Omotayo, A. E.; Simeon, O. M.; Amund, Olukayode O.The presence of coliforms in vegetables, water and soil samples from gardens in Lagos State was assayed for. The vegetables sampled were cabbage, waterleaf, carrot, lettuce and cucumber collected from five sites representing five geograpical zones. The isolates obtained were cultured on MacConkey (MAC) agar, Sorbitol MacConkey agar, Eosine Methylene Blue (EMB) agar and Salmonella-Shigella (SSA) agar. They were identified using morphological, biochemical and Analytical Profile Index 20E and 20SA kit. Hemolytic activity of the isolates was assayed for using Blood agar. The aerobic plate counts of soil, water and vegetable samples ranges from 8.80 x 107 to 8.00 x 109 cfu/g, 3.90 x 109 to 6.15 x 109 cfu/ml, and 3.30 x 109 to 1.08 x 1010 cfu/g, respectively. Coliforms were the predominant bacteria isolated from the sites. The coliform counts of cabbage, waterleaf, carrot, lettuce and cucumber were5. 01 x 109cfu/g, 6.76 x 107cfu/g, 5.49 x 107cfu/g, 1.58 x 108cfu/g and 4.67 x 104cfu/g respectively. The fecal colifom population range was between 2.51 x 103 and 1.31 x 108 cfu/g, while Salmonella and Shigella species ranged from 1.38 x 102-3.09 x 104 cfu/g. Escherichia coli 0157: H7 was not isolated from any of the sites. The study showed that washed vegetableswere contaminated with high microbial load especially coliforms.