Browsing by Author "Ugoji, E. O."
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Item Effects of waste engine oil spillage on soil physico-chemical and microbiological properties(1993) Amund, Olukayode O.; Omole, C. A.; Esiobu, N.; Ugoji, E. O.Changes in physic-chemical and microbial properties of soils contaminated with waste motor oil were monitored over a 24 week period. Oil application to soils resulted in a decrease in moisture content but brought about increase in organic matter, total nitrogen and available phosphorus contents. There was an initial decrease in microbial counts followed by a subsequent increase in population levels after four weeks. Microbial species diversity was however reduced in oil-contaminated sites relative to the control sites. Hydrocarbon-utilizing bacteria isolated from the experimental sites were identified as Pseudomonas, Acinetobacter, Alcaligenes, Flavobacterium and Corynebacterium. The organisms grew on long-chain n-alkanes, crude oil and fresh engine oil while a few species grew on aromatic hydrocarbons. Laboratory biodegradation studies of fresh engine oil using strains of Pseudomonas, Acinetobacter, and Corynebacterium showed a progressive decrease in oil concentration and pH of the medium due to the production of acidic metabolites.Item Occurence and Characteristics of HydroCarbon-Utilizing Bacteria in Nigerian Soils Contaminated with Spent Motor Oil(1987) Amund, Olukayode O.; Adebowale, A. A.; Ugoji, E. O.Soil samples were collected from four different oil-contaminated sites in Lagos, Nigeria, Enrichment of soil samples in a mineral salts medium containing SAE 40 motor oil as carbon source resulted in the isolation of eleven bacterial species. These organisms were identified as species of Corynebacterium, Acinetobacter, Pseudomonas, Bacillus, Arthrobacter, Gemella, Flavobacterium and Micrococcus. Substrate specificity tests showed that the organisms could utilize long chain n-alkanes including dodecane, tetradecane and hexadecane. Some species grew on some aromatic hydrocarbons, such as naphthalene, anthracene and phenanthrene. However, all the isolates grew on crude oil as well as motor oil. The growth of a Pseudomonas sp on motor oil resulted in the extensive degradation of the oil and in the synthesis of a new compound.Item Structural dynamics of microbial communities in polycyclic aromatic hydrocarbon-contaminated tropical estuarine sediments undergoing simulated aerobic biotreatment(Springer Berlin Heidelberg, 2017-05-01) Obi, Chioma C.; Adebusoye, Sunday A.; Amund, Olukayode O.; Ugoji, E. O.; Ilori, Matthew O.; Hedman, Curtis J; Hickey, William JCoastal sediments contaminated by polycyclic aromatic hydrocarbons (PAHs) can be candidates for remediation via an approach like land farming. Land farming converts naturally anaerobic sediments to aerobic environments, and the response of microbial communities, in terms of community structure alterations and corresponding effects on biodegradative activities, is unknown. A key goal of this study was to determine if different sediments exhibited common patterns in microbial community responses that might serve as indicators of PAH biodegradation. Sediments from three stations in the Lagos Lagoon (Nigeria) were used in microcosms, which were spiked with a mixture of four PAH, then examined for PAH biodegradation and for shifts in microbial community structure by analysis of diversity in PAH degradation genes and Illumina sequencing of 16S rRNA genes. PAH biodegradation was similar in all sediments, yet each exhibited unique microbiological responses and there were no microbial indicators of PAH bioremediation common to all sediments.