Browsing by Author "Wood, Christopher M."
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Item In vitro analysis of the bioavailability of six metals via the gastrointestinal tract of rainbow trout (Oncorhynchus mykiss)(Elsiever, 2007) Ojo, Adeola A.; Wood, Christopher M.An in vitro gut sac technique was used to compare the uptake rates of essential (copper, zinc and nickel) and non-essential metals (silver, cadmium and lead) at 50 mol L−1 each (a typical nutritive level in solution in chyme) in the luminal saline in four sections of the gastro-intestinal tract (stomach, anterior, mid and posterior intestines) of the freshwater rainbow trout. Cu, Zn, Cd and Ag exhibited similar regional patterns: on an area-specific basis, uptake rates for these metals were highest in the anterior intestine, lowest in the stomach, and approximately equal in the mid and posterior intestinal segments. When these rates were converted to a whole animal basis, the predominance of the anterior intestine increased because of its greater area, while the contribution of the stomach rose slightly to approach those of the mid and posterior intestines. However, for Pb and Ni, area-specific and whole organism transport rates were greatest in the mid (Pb) and posterior (Ni) intestines. Surprisingly, total transport rates did not differ appreciably among the essential and non-essential metals, varying only from 0.025 (Ag) to 0.050 nmol g−1 h−1 (Ni), suggesting that a single rate constant can be applied for risk assessment purposes. These rates were generally comparable to previously reported uptake rates from waterborne exposures conducted at concentrations 1–4 orders of magnitude lower, indicating that both routes are likely important, and that gut transporters operate with much lower affinity than gill transporters. Except for Ni, more metal was bound to mucus and/or trapped in the mucosal epithelium than was transported into the blood space in every compartment except the anterior intestine, where net transport predominated. Overall, mucus binding was a significant predictor of net transport rate for every metal except Cd, and the strongest relationship was seen for Pb.Item In vitro characterization of cadmium and zinc uptake via the gastrointestinal tract of rainbow trout (Oncorhynchus mykiss): interactive effects and the influence of calcium(Elsiever, 2008) Ojo, Adeola A.; Wood, Christopher M.An in vitro gut sac technique was employed to study whether Cd and Zn uptake mechanisms in the gastro-intestinal tract of the rainbow trout are similar to those at the gills, where both metals are taken up via the Ca transport pathway. Metal accumulation in surface mucus, in the mucosal epithelium, and transport into the blood space were assayed using radiolabelled Cd or Zn concentrations of 50 mol L−1 in the luminal (internal) saline. Elevated luminal Ca (10 or 100 mmol L−1 versus 1 mmol L−1) reduced Cd uptake into all three phases by approximately 60% in the stomach, but had no effect in the anterior, mid, or posterior intestine. This finding is in accordance with recent in vivo evidence that Ca is taken up mainly via the stomach, and that high [Ca] diets inhibit Cd accumulation from the food specifically in this section of the tract. In contrast, 10 mmol L−1 luminal Ca had no effect on Zn transport in any section, whereas 100 mmol L−1 Ca stimulated Zn uptake, by approximately threefold, into all three phases in the stomach only. There was no influence of elevated luminal Zn (10 mmol L−1) on Cd uptake in the stomach or anterior intestine, or of high Cd (10 mmol L−1) on Zn uptake in these sections. However, high [Zn] stimulated Cd transport into the blood space but inhibited accumulation in the mucosal epithelium and/or mucus-binding in the mid and posterior intestine, whereas high [Cd] exerted a reciprocal effect in the mid-intestine only. We conclude that Cd uptake occurs via an important Ca-sensitive mechanism in the stomach which is different from that at the gills, while Cd transport mechanisms in the intestine are not directly Ca-sensitive. Zn uptake does not appear to involve Ca uptake pathways, in contrast to the gills. These results are discussed in the context of other possible Cd and Zn transport pathways, and the emerging role of the stomach as an organ of divalent metal uptake.Item In vitro examination of interaction between copper and zinc uptake via the gastrointestinal tract of rainbow trout (Oncorhynchus mykiss).(Springer, 2009) Ojo, Adeola A.; Nadella, Sunita R.; Wood, Christopher M.An in vitro gut sac technique was used to investigate whether reciprocal inhibitory effects occurred between Cu and Zn uptake in the gastrointestinal tract of the rainbow trout and, if so, whether there was regional variation among the stomach, anterior intestine, mid intestine, and posterior intestine in the phenomena. Metal accumulation in surface mucus and in the mucosal epithelium and transport into the blood space were assayed using radiolabeled Cu or Zn at environmentally realistic concentrations of 50 μmol L−1 in the luminal saline, with 10-fold higher levels of the other metal (nonradioactive) as a potential inhibitor. Zn transport rates were generally higher than Cu transport rates in all compartments except the stomach, where they were lower. High [Zn] reduced Cu transport into the blood space in the mid and posterior intestines by 67% and 33%, respectively, whereas high [Cu] reciprocally reduced Zn transport into the blood space in these same sections by 54% and 78%. No inhibitions occurred in either the anterior intestine or the stomach. In these segments, elevated concentrations of the other metal stimulated Cu and Zn transport into the blood space and/or the mucosal epithelium by 50–100%, possibly by displacement from intracellular binding sites, thereby raising local concentrations at other transport sites. None of the treatments affected metal accumulation in surface mucus. The results indicate that one or more shared high-affinity pathways (possibly DMT1) occur in the mid and posterior intestine, which transport both Cu and Zn. These pathways appear to be absent from the stomach and anterior intestine, where other transport mechanisms may occur.