Prevalence and antibiotic resistance of bacteria isolated from ready-to-eat (RTE) food samples of highly patronized eateries in Ogbomoso-Oyo state, Nigeria
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Pelagia Research Library
Abstract
The study was designed to determine the prevalence and antibiotic resistance of bacterial
isolates of ready- to-eat-rice from four (4) of the major eateries in Ogbomoso, Oyo State,
Nigeria. General purpose and non-selective media were used for isolation and a total of ten (10)
bacteria were identified. Culture plates with less than 30 colonies and those with more than 300
colonies were removed and not included in the colony counts. The organisms obtained were
subsequently sub-cultured and subjected to various biochemical characterization tests for
identification. The organisms identified with their percentage of occurrence were Bacillus cereus
(30.4%), B. marcescens (4.3%), B. subtilis (4.3%), Streptococcus faecalis (4.3%), S. faecium
(13.0%), Staphylococcus aureus, (17.4%) Pseudomonas putida (4.3), Proteus vulgaris (4.3%),
Micrococcus luteus (13.0%) and M. acidiophilus (4.3%). Bacteria isolated were tested against
six (6) different antibiotics which are Ofloxacin OFL,(5μg); Erythromycin ERY, (10μg);
Gentamicin GEN, (10μg); Ceftriaxone CEF, (30μg); Ciprofloxacin CIP, (5μg); and
Cotrimoxazole COT, (50μg) in order to determine their susceptibility. Based on the overall
effectiveness, Ceftriaxone and Ofloxacin were the least active, while Cotrimoxazole was the most
active against the isolates.
Description
Staff Publication
Keywords
antibiotic,, resistance,, ready-to-eat-rice,, bacterial isolates