Hydrocarbon Degradation and Biosurfactant Production by an Acenaphthene-degrading Pseudomonas Species
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Date
2016-08
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis: Soil and Sediment Contamination: An International Journal
Abstract
An acenaphthene-degrading bacterium putatively identified as
Pseudomonas sp. strain KR3 and isolated from diesel-contaminated soil
in Lagos, Nigeria was investigated for its degradative and biosurfactant
production potentials on crude oil. Physicochemical analysis of the
sampling site indicates gross pollution of the soil with high
hydrocarbon content (2100 mg/kg) and detection of various heavy
metals. The isolate grew luxuriantly on crude oil, engine oil and
acenaphthene. It was resistant to septrin, amoxicillin and augmentin
but was susceptible to pefloxacin, streptomycin and gentamycin. It
was also resistant to elevated concentration of heavy metals such as
1–15 mM lead, nickel and molybdenum. On acenaphthene, the isolate
exhibited specific growth rate and doubling time of 0.098 day¡1 and
3.06 days, respectively. It degraded 62.44% (31.2 mg/l) and 91.78%
(45.89 mg/l) of 50 mg/l acenaphthene within 12 and 21 days. On crude
oil, the specific growth rate and doubling time were 0.375 day¡1 and
1.85 days with corresponding percentage degradation of 33.01%
(903.99 mg/l) and 87.79% (2403.71 mg/l) of crude oil (2738.16 mg/l)
within 9 and 18 days. Gas chromatographic analysis of residual crude
oil at the end of 18 days incubation showed significant reductions in
the aliphatic, alicyclic and aromatic fractions with complete
disappearance of benzene, propylbenzene, pristane, phytane, and
nC18-octadecane fractions of the crude oil. The isolate produced
growth-associated biosurfactant on crude oil with the highest
emulsification index (E24) value of 72% § 0.23 on Day 10 of incubation.
The partially purified biosurfactant showed zero tolerance for salinity
and had its optimal activity at 27 C and pH 2.0.
Description
Staff Publication
Keywords
biodegradation;, diesel oil-contaminated soil;, acenaphthene;, crude oil;, biosurfactant;, Pseudomonas
Citation
Lateef B. Salam, Oluwafemi S. Obayori & Obaje Hawa (2016): Hydrocarbon Degradation and Biosurfactant Production by an Acenaphthene-degrading Pseudomonas Species, Soil and Sediment Contamination: An International Journal, DOI: 10.1080/15320383.2016.1217826