Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies
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Date
2018-04
Journal Title
Journal ISSN
Volume Title
Publisher
PLoS Neglected Tropical Diseases
Abstract
Background
Metabolic fingerprinting analysis can offer insights into underlying reactions in a biological
system; hence it is crucial to the understanding of disease pathogenesis and could provide
useful tools for discovering biomarkers. We sought to examine the urine and plasma metabolome
in individuals affected by urogenital schistosomiasis and its associated-bladder
pathologies.
Methodology
Blood and midstream urine were obtained from volunteers who matched our inclusion criteria
among residents from Eggua, southwestern Nigeria. Samples were screened by urinalysis,
microscopy, PCR and ultrasonography, and categorised as advanced (urogenital
schistosomiasis associated-bladder pathologies), infection-only (urogenital schistosomiasis
alone) and controls (no infection and no pathology). Metabolites were extracted and data
acquired with ultra high-performance liquid chromatography coupled with Thermo Q-Exactive
orbitrap HRMS. Data was analysed with MetaboAnalyst, Workflow4Metabolomics,
HMDB, LipidMaps and other bioinformatics tools, with univariate and multivariate statistics
for metabolite selection.
Principal findings
There were low levels of host sex steroids, and high levels of several benzenoids, catechols
and lipids (including ganglioside, phosphatidylcholine and phosphatidylethanolamine), in
infection-only and advanced cases (FDR<0.05, VIP>2, delta>2.0). Metabolites involved in
biochemical pathways related to chorismate production were abundant in controls, while
those related to choline and sphingolipid metabolism were upregulated in advanced cases (FDR<0.05). Some of these human host and Schistosoma haematobium molecules, including
catechol estrogens, were good markers to distinguish infection-only and advanced
cases.
Conclusions
Altered glycerophospholipid and sphingolipid metabolism could be key factors promoting
the development of bladder pathologies and tumours during urogenital schistosomiasis.
Description
Staff Publication
Keywords
Citation
Adebayo AS, Mundhe SD, Awobode HO, Onile OS, Agunloye AM, Isokpehi RD, et al. (2018) Metabolite profiling for biomarkers in Schistosoma haematobium infection and associated bladder pathologies. PLoS Negl Trop Dis 12(4): e0006452. https://doi.org/10.1371/journal.pntd.0006452https://doi.org/10.1371/journal.pntd.0006452