Genotoxicity Evaluation of Landfill Solid Leachates from Waste Dumpsite in Lagos State, Nigeria Using the Allium cepa Assay
Loading...
Date
2018-04
Journal Title
Journal ISSN
Volume Title
Publisher
Plant and Animal Research Journal
Abstract
The management of municipal landfill’s leachate is considered one of the most
significant environmental issues. Potential mutagenic and genotoxic effects of
solid waste leachates (SWL) were investigated using the Allium cepa root meristematic cells assay technique. In Allium root growth inhibition test, experimental onion bulbs were cultivated in various concentrations of the SWL and
distilled water was used as a negative control. The root tips from the treated bulb
were processedby orcein squash technique after 72 h. The mean lengths of root
bundles were obtained andeffective concentration (EC) values calculated. Some
of the physicochemical properties of the WL were also determined. The A. cepa
assay was carried out at concentrations of 0.05, 0.1, 2.0, 5, 10, 20, 50 and 100%
of the WL. There was a statistically different (p < 0.05), concentrationdependent inhibition of onion root growth and mitotic index, and induction of
chromosomal aberrations in the onion test. The results of the physicochemical
analysis revealed that the concentrations of some parameters were above the
maximum permissible limit set by the world health organization (WHO) and
could partly be correlated with the toxicity of wastewater. The landfill leachates
pose a risk to human health and the environment in general. Hence, ecotoxicity/genotoxicity assays would be useful in leachate risk assessment when coupled
with physicochemical analysis.
Description
Keywords
Genotoxicity, chromosomal aberration, solid waste leachates, mitotic index, Allium cepa
Citation
Ogunyemi AK, Samuel TA, Amund OO, Ilori MO (2018) Genotoxicity Evaluatioan of Landfill Solid Waste Leachates from a Waste Dumpsite in Lagos State, Nigeria Using the Allium cepa Assay. Plant and Animal Research Journal 1 (3): 68 – 76. doi: 10.11594/parj.01.03.02