Carbazole angular dioxygenation and mineralization by bacteria isolated from hydrocarbon-contaminated tropical African soil
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Date
2014-08-01
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Berlin Heidelberg
Abstract
Four bacterial strains isolated from hydrocarbon-contaminated soils in Lagos, Nigeria, displayed
extensive degradation abilities on carbazole, an N-heterocyclic aromatic hydrocarbon.
Physicochemical analyses of the sampling sites (ACPP, MWO, NESU) indicate gross pollution
of the soils with a high hydrocarbon content (157,067.9 mg/kg) and presence of heavy metals.
Phylogenetic analysis of the four strains indicated that they were identified as Achromobacter sp.
strain SL1, Pseudomonas sp. strain SL4, Microbacterium esteraromaticumstrain SL6,
and Stenotrophomonas maltophilia strain BA. The rates of degradation of carbazole by the four
isolates during 30 days of incubation were 0.057, 0.062, 0.036, and 0.050 mg Lā1 h
ā1 for strains
SL1, SL4, SL6, and BA. Gas chromatographic (GC) analyses of residual carbazole after 30 days
of incubation revealed that 81.3, 85, 64.4, and 76 % of 50 mg l
ā1 carbazole were degraded by
strains SL1, SL4, SL6, and BA, respectively. GC-mass spectrometry and high-performance liquid
chromatographic analyses of the extracts from the growing and resting cells of strains SL1, SL4,
and SL6 cultured on carbazole showed detection of anthranilic acid and catechol while these
metabolites were not detected in strain BA under the same conditions. This study has established
for the first time carbazole angular dioxygenation and mineralization by isolates from African
environment.
Description
Keywords
Biodegradation, Carbazole, Achromobacter, Stenotrophomonas, Microbacterium, Pseudomonas
Citation
Salam, L. B., Ilori, M. O., Amund, O. O., Numata, M., Horisaki, T., & Nojiri, H. (2014). Carbazole angular dioxygenation and mineralization by bacteria isolated from hydrocarbon-contaminated tropical African soil. Environmental Science and Pollution Research, 21(15), 9311-9324.