Biodegradation of petroleum hydrocarbons in the presence of nickel and cobalt
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Date
2013-11
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of basic microbiology
Abstract
Bioremediation of environments co-contaminated with hydrocarbons and heavy metals often pose
a challenge as heavy metals exert toxicity to existing communities of hydrocarbon degraders.
Multi-resistant bacterial strains were studied for ability to degrade hydrocarbons in chemically
defined media amended with 5.0 mM Ni2þ, and Co2þ. The bacteria, Pseudomonas aeruginosa
CA207Ni, Burkholderia cepacia AL96Co, and Corynebacterium kutscheri FL108Hg, utilized crude oil and
anthracene without lag phase at specific growth rate spanning 0.3848–0.8259 per day. The
bacterial populations grew in hydrocarbon media amended with nickel (Ni) and cobalt (Co) at
0.8393–1.801 days generation time (period of exponential growth, t ¼ 15 days). The bacteria
degraded 96.24–98.97, and 92.94–96.24% of crude oil, and anthracene, respectively, within
30 days without any impedance due to metal toxicity (at 5.0 mM). Rather, there was reduction of
Ni and Co concentrations in the axenic culture 30 days post-inoculation to 0.08–0.12 and 0.11–
0.15 mM, respectively. The metabolic functions of the bacteria are active in the presence of toxic
metals (Ni and Co) while utilizing petroleum hydrocarbons for increase in biomass. These findings
are useful to other baseline studies on decommissioning of sites co-contaminated with
hydrocarbons and toxic metals.
Description
Keywords
Biodegradation, Nickel, Cobalt, Heavy metal, Crude oil, Anthracene, Petroleum hydrocarbon, Bacteria
Citation
Oyetibo, G. O., Ilori, M. O., Obayori, O. S., & Amund, O. O. (2013). Biodegradation of petroleum hydrocarbons in the presence of nickel and cobalt. Journal of basic microbiology, 53(11), 917-927.