Intra- and Inter-individual Variation in Population of Lactobacillus and Bifidobacterium in Faeces of Some Healthy Individuals
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Date
2015-12-04
Journal Title
Journal ISSN
Volume Title
Publisher
British Microbiology Research Journal
Abstract
Aims: To evaluate the variation of Lactobacillus and Bifidobacterium population within and
between healthy individuals.
Methodology: Five healthy participants were recruited, and advised not to consume yoghurt,
antibiotics, alcohol and steroid during the study. Three (3) fecal samples from each participant
obtained at one week interval (total of 15 samples a week) for three weeks were examined for each
subject bacteria. The samples were collected in sterile specimen jars and immediately taken to the
laboratory for analysis. MRS and BIM-25, a selective medium were used for the enumeration of
Lactobacillus sp and Bifidobacteria respectively using plate count method. Target bacteria were
confirmed by PCR technique and biochemical tests. Results: All isolates picked from their respective selective media were confirmed to be
lactobacillus and bifidobacteria. The confirmation was carried out using both biochemical tests and
PCR. The results of the statistical analysis of the data obtained using SPSS version 16 showed that
intra-individual variation of the population of Lactobacillus and Bifidobacteria was significantly lower
than inter-individual variation at P <0.05.
Conclusion: This study establishes the fact that the composition level of Lactobacillus and
Bifidobacterium varies within and between healthy human gut at different points in time.
Description
Staff Publication
Keywords
Gastrointestinal tract;, Lactobacillus;, Bifidobacteria;, variation;, population;, microbiota;, probiotics.