Sustainable generation of bioethanol from sugarcane wastes by Streptomyces coelicolor strain COB KF977550 isolated from a tropical estuary
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Date
2021-01-20
Journal Title
Journal ISSN
Volume Title
Publisher
Scientific African
Abstract
The damaging effect and challenges associated with the use of fossil fuel is enormous and very
costly. Biofuels could be obtained from plant biomass wastes which are known to be sources of
environmental pollution and breeding grounds for vectors of diseases. Sugarcane bagasse was
exploited as a renewable substrate for obtaining bioethanol using Streptomyces strain COB
KF977550 as inoculum. Submerged aerobic batch fermentation was performed in flasks
containing mineral salts medium supplemented with 5.0 g (w/v) sugarcane bagasse. Incubation
was done in a shaker (150 rpm) at 30 oC for 21 days. Microbial growth was assessed by
measurement of the optical density (O.D 600nm) at 3-day intervals. Fractional distillation was
carried out in batch mode using a simple fractional distillation setup. Metabolic products were
determined using GC-FID. Further analyses were performed using FTIR and GC-MS. The
optical density of S.coelicolor strain COB KF977550 increased from 0.9 to 1.41. The GC-FID
showed that 43.08 g/L ethanol was generated. Interestingly, the results showed the presence of
diverse biochemicals released into the medium in addition to the main product ethanol. Ten
carboxylic acids including formic acid, glycolic acid, tartaric acid, acetic acid, citric acid, oxalic
acid, malic acid, lactic acid, n-valeric acid, and 3-hydroxybutyric acid were identified as
biochemical organic acids by-products.
Description
Keywords
Bioethanol;, Sugarcane bagasse;, Biomolecules;, Lignocellulose wastes;, Streptomyces.
Citation
Olanike Maria Buraimoh , Adewale Kayode Ogunyemi , Chukwuemeka Isanbor , Oluwafemi Segun Aina , Olukayode Oladipo Amund , Mathew Olusoji Ilori , Oluwole Babafemi Familoni (2021). Sustainable generation of bioethanol from sugarcane wastes by Streptomyces coelicolor strain COB KF977550 isolated from a tropical estuary, Scientific African. doi: https://doi.org/10.1016/j.sciaf.2021.e00709