Partial Purification and Characterization of Cellulolytic Enzyme from Bacillus pantothenticus Isolated from a Dumpsite
Loading...
Date
2017-04-27
Journal Title
Journal ISSN
Volume Title
Publisher
Research & Reviews: Journal of Microbiology and Biotechnology
Abstract
Cellulose hydrolyzing organism, Bacillus pantothenticus, was
isolated from a dumpsite soil. Partial purification of the enzyme with
precipitation with 60% chilled acetone and on Biogel P-100 gel
filtration yielded an enzyme with specific activity of 253.50, 10.40%
yield and purification fold of 2.41. The apparent Km and Vmax for
Carboxymethylcellulose (CMC) hydrolysis was 1.167 mg/ml and 0.833
μg of glucose/ml/min respectively. The native molecular weight of
the enzyme from B. pantothenticus was estimated as 51.48 kDa. The
cellulase produced showed activity over broad range of temperature
(30-70°C) with maximum activity at 60°C. The enzyme also showed
activity in a wide pH range of 4-11 with optimum activity at pH 4.5.
At concentration of 10 mM, KCl, MgCl2, NaCl and NiCl2 inhibited the
enzyme while CoCl2 activated the enzyme. The enzyme showed highest
activity with microcrystalline cellulose as substrate followed by CMC.
Significant activity was also observed with organic substrates such as
sugarcane bagasse, orange bagasse and corn cub. The study concluded
that the cellulose enzyme obtained had suitable catalytic properties for
use in biodegradation of waste and other industrial applications.
Description
Staff Publication
Keywords
Cellulase;, Bacillus pantothenticus; Purification characterization, Purification characterization
Citation
Quadri, H.O., Ademakinwa, A.N., Adejumo, A.L., & Agboola, F. (2017). Partial Purification and Characterization of Cellulolytic Enzymefrom Bacillus pantothenticus Isolated from a Dumpsite. Research and Reviews: Journal of Microbiology and Biotechnology, 6, 4-11.