Kinetic and thermodynamic investigations of cell-wall degrading enzymes produced by Aureobasidium pullulans via induction with orange peels: application in lycopene extraction
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Date
2019-08-09
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis Online
Abstract
The production of cell-wall degrading enzymes (CWDE) such as cellulase and pectinase by
Aureobasidium pullulans NAC8 through induction using orange peels was investigated for the
potential application of these enzymes in the extraction of lycopene from tomato skin, waste, and
paste (SWP). The CWDE was then immobilized via entrapment in alginate beads for lycopene
extraction and the kinetic/thermodynamic properties of the free and immobilized CWDE investigated.
The optimum production of CWDE occurred at pH, temperature, and orange peel concentration
of 6.0, 50 C, and 2.0% (w/v), respectively. The values obtained for some kinetic and
thermodynamic parameters such as E
d; t1=2; DG
d; and DH
d indicate that both free and immobilized
cellulase and pectinase were thermostable between 40 and 50 C. Maximum lycopene
extracted from the tomato SWP was 80 ± 2.4 mg/kg, 42 ± 1.3 mg/kg and 60 ± 1.2 mg/kg, respectively,
using the immobilized CWDE. The entrapped CWDE was able to extract lycopene with yields
of 58 ± 4.2, 51 ± 1.2 and 57 ± 4.2% for tomato SWP respectively after the fifth cycle. Using orange
peels for the induction of CWDE by A. pullulans offers a unique and cheaper approach to obtaining
thermostable multi-enzyme complexes employable for easy lycopene extraction from
tomato SWP.
Description
Staff Publication
Keywords
Cellulase;, pectinase;, lycopene;, submerged fermentation;, Aureobasidium pullulans NAC8;, orange peel; tomatoes
Citation
Adedeji Nelson Ademakinwa & Femi Kayode Agboola (2019): Kinetic and thermodynamic investigations of cell-wall degrading enzymes produced by Aureobasidiumpullulans via induction with orange peels: application in lycopene extraction, Preparative Biochemistry and Biotechnology, DOI: 10.1080/10826068.2019.1650375