A Comparative Study of Biosurfactant Synthesis by Pseudomonas aeruginosa Isolated from Clinical and Environmental Samples

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dc.contributor.authorAgwu, O. A.
dc.contributor.authorIlori, Matthew O.
dc.contributor.authorAdebusoye, S. A.
dc.contributor.authorAmund, Olukayode O.
dc.date.accessioned2019-06-25T17:24:53Z
dc.date.available2019-06-25T17:24:53Z
dc.date.issued2012-02-15
dc.description.abstractEvaluation of emulsifying activities indicates that biosurfactants were produced by an environmental (strain EP1) and a clinical (strain CP1) species of Pseudomonas aeruginosa. During growth on hydrocarbons, the organisms produced biosurfactants. Both strains grew luxuriantly on motor oil and readily synthesized abundant surfactants at the expense of easily metabolizable substrates. During a 12-day cultivation on motor oil, the organisms produced growth-associated extracellular surfactants with emulsification activities of 71 and 38% for EP1 and CP1, respectively. The generation times obtained for EP1 and CP1 were 1.74 and 2.66 days. The biosurfactants that could not be secreted on glucose were partially purified and putatively identified as rhamnolipids. The surface-active compounds present high emulsification activity and stability in the pH range of 3.0–10.0, temperature range of 4°C–100°C, and salinity range of 16–44% and are capable of stabilizing oil-in-water emulsions with several hydrocarbons. Typical emulsions produced were stable for several weeks. The results also showed that the biosurfactants were able to remove a significant amount of crude oil from contaminated soil; for instance, strain EP1 surfactant removed 54%, CP1 41%, detergent 42%, and water 30%. The rhamnolipids from these strains represent a new class of biosurfactants that have potential for use in a variety of biotechnological and industrial applications where extremes of pH, thermal, and saline conditions would have little or no effect on activity.en_US
dc.identifier.citationAgwu, O. A., Ilori, M. O., Adebusoye, S. A., & Amund, O. O. (2012). A Comparative study of biosurfactant synthesis by Pseudomonas aeruginosa isolated from clinical and environmental samples. Petroleum Science and Technology, 30(5), 503-517.en_US
dc.identifier.urihttps://doi.org/10.1080/10916466.2010.487269
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/156
dc.language.isoenen_US
dc.publisherTaylor & Francis Groupen_US
dc.subjectBiodegradationen_US
dc.subjectBiosurfactanten_US
dc.subjectEmulsification indexen_US
dc.subjectHydrocarbonsen_US
dc.subjectRhamnolipiden_US
dc.titleA Comparative Study of Biosurfactant Synthesis by Pseudomonas aeruginosa Isolated from Clinical and Environmental Samplesen_US
dc.typeArticleen_US
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