Browsing by Author "Michel Jr, Frederick C."
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item Assessment of bacterial degradation of lignocellulosic residues (sawdust) in a tropical estuarine microcosm using improvised floating raft equipment(Elsevier, 2015-10-01) Buraimoh, Olanike M.; Ilori, Matthew O.; Amund, Olukayode O.; Michel Jr, Frederick C.; Grewal, Sukhbir K.In situ and laboratory studies were carried out to determine the ability of bacterial strains isolated from a tropical lagoon to degrade lignin and carbohydrate components of sawdust, with a view to abating the impact of sawdust pollution on these ecosystem. A floating raft system was designed and fabricated to carry out the in situ biodegradation studies over a period of 24 weeks. Nine bacterial strains identified by 16S rRNA gene sequencing as species of Streptomyces, Bacillus and Paenibacillus isolated from the lagoon were used as seed organisms. In the in situ study, 59.2% of sawdust was depleted at the rate of 1.175 x 10-4 gd-1 cm-3 by the bacterial isolates, whereas the lignin component of the sawdust decreased by up to 82.5% at the rate of 1.80 x 10-5 gd-1 cm-3. The maximum decrease in carbohydrate content was 85% at the rate of 2.192 x 10-7 gd-1 cm-3. In a similar experiment under laboratory conditions, total weight losses ranging from 26 to 51% in the wood residues were observed.Item The degradation of coniferyl alcohol and the complementary production of chlorogenic acids in the growth culture of Streptomyces albogriseolus KF977548 isolated from decaying wood residues(Elsevier, 2017-01-01) Buraimoh, Olanike M.; Ilori, Matthew O.; Amund, Olukayode O.; Isanbor, Chukwuemeka; Michel Jr, Frederick C.Coniferyl alcohol is one of the major precursors of lignin; the most abundant aromatic compound and a natural resource currently receiving attention because of the value-added metabolites resulting from its degradation. Growth study of Streptomyces albogriseolus KF977548 (strain AOB) isolated from decaying wood residues in a tropical estuarine ecosystem was carried out using coniferyl alcohol as a sole carbon source. Cell growth and metabolite production were monitored at 24 h interval by dry weight measurements and HPLC, LC–MS-DAD analyses. Biochemical and PCR assays were carried out to detect the major catabolic enzymes of interest. Strain AOB utilized coniferyl alcohol completely within 72 h ( = 0.204 h−1, Td = 3.4 h). Laccase and peroxidase were released into the growth medium up to 0.099 and 98 mol/mL respectively. Protocatechuate 3, 4-dioxygenase and demethylase were detected in the genome whilst ortho-adipate pathway was clearly indicated. Growth on coniferyl alcohol or caffeic acid as mono substrates resulted in the production of secondary metabolites identified by HPLC–MS as 1- caffeoylquinic and 3,4,5-tricaffeoylquinic acids, known as chlorogenic acids, in the culture medium. The microbial production of chlorogenic acids from a lignin-related substrate base by strain AOB could arouse a plausible biotechnological process.