Detection of carbohydrate-active enzymes and genes in a spent engine oil-perturbed agricultural soil

Simple item page
dc.contributor.authorSalam, Lateef B.
dc.date.accessioned2021-09-13T10:35:00Z
dc.date.available2021-09-13T10:35:00Z
dc.date.issued2018-08
dc.descriptionStaff Publicationen_US
dc.description.abstractBackground: The purpose of this study is to decipher the diverse carbohydrate metabolism pathways in a spent engine oil-perturbed agricultural soil, enunciate the carbohydrate-active enzymes and genes involved in the process, taxonomically classify the annotated enzymes and genes, and highlight the importance of the study for ecological and biotechnological processes. Results: Functional analysis of the metagenome of spent engine oil (SEO)-contaminated agricultural soil (AB1) using the Kyoto Encyclopedia of Genes and Genomes (KEGG) GhostKOALA, Cluster of Orthologous Groups (COG) of proteins, the Carbohydrate-Active Enzymes (CAZy) database, and the NCBI’s conserved domain database (CDD) revealed extensive metabolism of carbohydrates via diverse carbohydrate-active enzymes and genes. Enzymes and genes annotated for glycolysis/gluconeogenesis pathway, citric acid (TCA) cycle, pentose phosphate pathway, and pyruvate metabolism, among others, were detected, and these were not detected in the original agricultural soil (1S). Analysis of carbohydrate-active enzymes, using the CAZy database, showed 45 CAZy families with preponderance of glycoside hydrolases (GHs, 46.7%), glycosyltransferases (GTs, 24.4%), and carbohydrate-binding modules (CBMs, 15.5%). Taxonomic classification of the annotated enzymes and genes for carbohydrate metabolism using the GhostKOALA and CAZy databases revealed the predominance of the phylum Proteobacteria with the representative genera Pseudomonas (18%), Sphingobium (13.5%), and Sphingomonas (4.5%), respectively. Biotechnologically important enzymes such as xylanases, endoglucanases, α- and β-glucosidases and glycogen debranching enzymes were also retrieved from the metagenome. Conclusions: This study revealed the presence of diverse carbohydrate-active enzymes and genes mediating various carbohydrate metabolism pathways in the SEO-perturbed soil metagenome. It also reveals the detection of biotechnologically important enzymes with potentials for industrial use.en_US
dc.identifier.citationSalam, L. B. (2018). Detection of carbohydrate-active enzymes and genes in a spent engine oil-perturbed agricultural soil. Bulletin of the National Research Centre, 42(1). doi:10.1186/s42269-018-0013-6en_US
dc.identifier.urihttps://doi.org/10.1186/s42269-018-0013-6
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/1236
dc.language.isoenen_US
dc.publisherBulletin of the National Research Centreen_US
dc.subjectSpent engine oil,en_US
dc.subjectAgricultural soil,en_US
dc.subjectSoil microcosm,en_US
dc.subjectIllumina sequencing,en_US
dc.subjectCarbohydrate metabolism,en_US
dc.subjectCarbohydrate-active enzymes,en_US
dc.subjectMicrobial enzymes and genesen_US
dc.titleDetection of carbohydrate-active enzymes and genes in a spent engine oil-perturbed agricultural soilen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Salam2018_Article_DetectionOfCarbohydrate-active.pdf
Size:
4.76 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles Biological Sciences