Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8
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Date
2017-07-04
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Strain improvement of a low fructosyltransferase-producing Aureobasidium pullulans
NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide
and ethyl methane sulfonate. The wild-type and mutant strain were distinguished using Random
amplified polymorphic DNA PCR and DNA fingerprinting analysis. Plackett-Burman and
Box Behnken design were statistical tools used to determine important media parameters and optimization,
respectively. Phenotypically and genetically, the new improved strain was different from
the wild-type. The most important media parameters from PDB influencing fructosyltransferase
production were ammonium chloride, sucrose and yeast extract at p<0.05. Some significant
parameters obtained with the BBD exhibited quadratic effects on FTase. The F values (35.37
and 32.11), correlation coefficient (0.98 and 0.97) and the percent coefficient of variation (2.53%
and 2.40%) were obtained for extracellular and intracellular FTase respectively. The validation
of the model in the improved strain resulted in an overall 6.0 and 2.0-fold increase in extracellular
and intracellular FTase respectively compared to the wild-type.
A relatively low FTase-producing strain of Aureobasidium pullulans NAC8 was enhanced for
optimum production using a two-pronged approach involving mutagenesis and statistical optimization.
The improved mutant strain also had remarkable biotechnological properties that make it a
suitable alternative than the wild-type
Description
Staff Publication
Keywords
Mutagenesis;, Plackett-Burman design;, DNA fingerprinting RAPDPCR;, Box-Behnken design; RSM, RSM
Citation
Ademakinwa, A.N., Ayinla, Z.A., & Agboola, F. (2017). Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8. Journal of Genetic Engineering & Biotechnology, 15, 345 - 358.