Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8
| dc.contributor.author | Ademakinwa, Adedeji Nelson | |
| dc.contributor.author | Ayinla, Zainab Adenike | |
| dc.contributor.author | Agboola, Femi Kayode | |
| dc.date.accessioned | 2021-02-16T14:41:34Z | |
| dc.date.available | 2021-02-16T14:41:34Z | |
| dc.date.issued | 2017-07-04 | |
| dc.description | Staff Publication | en_US |
| dc.description.abstract | Strain improvement of a low fructosyltransferase-producing Aureobasidium pullulans NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide and ethyl methane sulfonate. The wild-type and mutant strain were distinguished using Random amplified polymorphic DNA PCR and DNA fingerprinting analysis. Plackett-Burman and Box Behnken design were statistical tools used to determine important media parameters and optimization, respectively. Phenotypically and genetically, the new improved strain was different from the wild-type. The most important media parameters from PDB influencing fructosyltransferase production were ammonium chloride, sucrose and yeast extract at p<0.05. Some significant parameters obtained with the BBD exhibited quadratic effects on FTase. The F values (35.37 and 32.11), correlation coefficient (0.98 and 0.97) and the percent coefficient of variation (2.53% and 2.40%) were obtained for extracellular and intracellular FTase respectively. The validation of the model in the improved strain resulted in an overall 6.0 and 2.0-fold increase in extracellular and intracellular FTase respectively compared to the wild-type. A relatively low FTase-producing strain of Aureobasidium pullulans NAC8 was enhanced for optimum production using a two-pronged approach involving mutagenesis and statistical optimization. The improved mutant strain also had remarkable biotechnological properties that make it a suitable alternative than the wild-type | en_US |
| dc.identifier.citation | Ademakinwa, A.N., Ayinla, Z.A., & Agboola, F. (2017). Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8. Journal of Genetic Engineering & Biotechnology, 15, 345 - 358. | en_US |
| dc.identifier.uri | DOI:10.1016/j.jgeb.2017.06.012 | |
| dc.identifier.uri | http://repository.elizadeuniversity.edu.ng/handle/20.500.12398/844 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.subject | Mutagenesis; | en_US |
| dc.subject | Plackett-Burman design; | en_US |
| dc.subject | DNA fingerprinting RAPDPCR; | en_US |
| dc.subject | Box-Behnken design; RSM | en_US |
| dc.subject | RSM | en_US |
| dc.title | Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by Aureobasidium pullulans NAC8 | en_US |
| dc.type | Article | en_US |