Browsing by Author "Asaolu, M. F."

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    Biochemical changes associated with sickle cell anaemia
    (Biosciences Biotechnology Research Asia, 2015-05-02) Asaolu, M. F.; Oyeyemi, A. O.; Fakunle, Julius B.; Ajose, A. O.
    Some blood chemical parameters including some enzyme activities were determined in forty two sickle cell patients in Ekiti state, Nigeria. All the parameters of interest analyzed for were found present in all the samples. The mean values for uric acid (0.35±0.16 mol/l), creatinine (100.55±8.13 μmol/l), urea (10.50±6.28 mmol/l), Total bilirubin (14.42±2.03 μmol/l), conjugated bilirubin (10.53±2.67 μmol/l), alkaline phosphatase (82.67±10.87 μ/l) aspartate transaminase (SGOT)(20.68±9.85 μ/l) and alanine transaminase (SGPT)(28.68±9.85 μ/L) were significantly higher (P< 0.05) in sickle cell patients than the control. Serum sodium (Na+)(110.00±4.0 lmmol/L), potassium (K+)(2.56±0.63 mmol/L) total protein (60.05±10.35 g/L), albumin (30.16±3.28 g/L), Calcium (2.00±0.1 mmol/L) and inorganic phosphate (1.12±0.19 mmol/L) were also found to be significantly lowered in sickle cell patient when compared with the control. Analysis of the results showed an increase in plasma chloride (99.33±2.54 mmol/L) and decrease in bicarbonate (22.99±2.06 mmol/L). The differences were however not statistically significant (P> 0.05). The significant raised level of SGPT, SGOT, ALP, total bilirubin, conjugated bilirubin, and significant lower levels of total protein, albumin, calcium, inorganic phosphate indicates that there may be abnormal liver and renal function as well as muscular and bone dysfunction in these patients.
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    A comparative study of serum ferritin and other acute phase reactants (APR) in minor and major surgery
    (Academic Journals, 2009-12-31) Asaolu, M. F.; Oyeyemi, A. O.; Fakunle, Julius B.
    This study was carried out on serum ferritin, acute phase reactants (APR), iron (Fe) and total iron binding capacity (TIBC) in healthy women in Ado Ekiti, Nigeria and in female patients undergoing suture (minor operation) and ceaserean operations at the Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, Nigeria between February and March, 2009. The results revealed post-operative serum ferritin and APR concentrations to be significantly higher (p> 0.01) while iron and TIBC were significantly lower in patients undergoing caesarean operation compared to both patients with minor operation and healthy women. The pre-operative results of serum ferritin were however significantly lower in patient undergoing major operation than in patients with minor operation. The present study was carried out to examine the changes in serum ferritin and other acute phase reactants in response to the magnitude of tissue damage. It can be concluded that the concentrations of serum ferritin and acute phase reactant increases as the magnitude of tissue damages increases. There is an acute phase response of serum ferritin levels following major surgery.
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    Cross-sectional study of antioxidant status in normotensive and hypertensive pregnancy
    (Tropical Journal of Obstetrics and Gynaecology, 2011) Owolabi, A. T.; Marcel, T. T.; Fakunle, Julius B.; Togun, R. A.; Akinola, N. O.; Asaolu, M. F.; Fasubaa, O. B.; Bisiriyu, L. A.; Kuti, O.
    Objectives: The study measured the concentrations of antioxidants in women during pregnancy and the Post Partum Period (PPP) with a view to investigating their role in the aetiology of pregnancy induced hypertension (PIH). Study Design: Informed consent was obtained from 105 women who were divided into three groups: 15 age and parity matched normotensive non-pregnant (control group), 45 normotensive and 45 hypertensive pregnant women. The two groups of pregnant women were divided into three groups of 15 each and studied in the second and third trimesters and PPP respectively. Venous blood was obtained from all the participants for measurements of some antioxidants (uric acid, albumin, catalase and vitamin C). Data were analyzed using descriptive and inferential statistical methods. Results: The results show that the mean concentrations of catalase, albumin, vitamin C and uric acid for the control were 3.1 ± 0.18 µ/l, 46.6 ± 6.6 g/l, 0.38 ± 0.01 mg/dl and 0.16 ± 0.03 mmol/l respectively. The mean concentration of all antioxidants except uric acid were significantly lower during pregnancy when compared with controls (t= 2.06; p<0.01). In the normotensive group of pregnant women, vitamin C was the only antioxidant that showed significant higher concentration when the second trimester concentration and third trimester concentration were compared (t=2.06; p<0.05). Uric acid levels were significantly higher (t=2.06; p<0.05) and nd rd catalase and vitamin C levels were significantly lower in the hypertensive group during the 2 and 3 trimesters (t=2.06; p<0.05). There was a tendency for all antioxidant concentrations to return to normal values during the PPPin the normotensive group; however in the hypertensive group, uric acid levels remained significantly higher (t=2.06; p<0.05). Conclusion: In conclusion this study showed that pregnancy generally reduced the concentration of antioxidants but vitamin C levels were higher in late pregnancy of normotensive women. Therefore higher levels of vitamin C may protect against PIH.
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    Evaluation of in-vitro antioxidant activities of methanol extracts of Persea americana and Cnidosculus aconitifolius
    (Pak J Nutr, 2010) Asaolu, M. F.; Asaolu, S. S.; Fakunle, Julius B.; Emman-Okon, B. O.; Ajayi, E. O.; Togun, R. A.
    The present study was undertaken to investigate the antioxidant activities of Persea americana and Cnidosculous aconitifolius. The in-vitro antioxidant activity of the methanol extracts of the leaves of Persea americana and Cnidosculous aconitifolius was evaluated using various experimental methods such as 1,1-diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide and reducing power radical-scavenging activity assay. Phytochemical screening as well as the amounts of total phenol and flavonoids were also determined. The present study revealed that both the methanol extracts of the leaves of Persea americana and Cnidosculous aconitifolius possess significant antioxidant activities. However, Persea americana was found to have higher radical scavenging activity than Cnidosculous aconitifolius but the phenol content of Cnidosculous aconitifolius was higher than that of Persea americana whereas Persea americana was observed to possess more flavonoids than Cnidosculous aconitifolius.