Research Articles in Physical and Chemical Sciences

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3-D Seismic Interpretation and Volumetric Estimation of “Osaja Field” Niger Delta, Nigeria
(International Letters of Natural Sciences, 2016) Lawson-Jack, Osaki; Iheanyichukwu, Opara A.; Njoku, Okereke C.; Petters, Adiela U.; Onyema, Njoku I.; Terhemba, Emberga T.; Ndidiamaka, Eluwa
3-D seismic interpretation and petrophysical analysis of the Osaja Field, Niger Delta, was carried out with aim of carrying out a detailed structural interpretation, reservoir characterization and volumetric estimation of the field. Four wells were correlated across the field to delineate the lithology and establish the continuity of reservoir sand as well as the general stratigraphy of the area. The petrophysical analysis carried out, revealed two sand units that are hydrocarbon bearing reservoirs (Sand_A and Sand_B).The spatial variation of the reservoirs were studied on a field wide scale using seismic interpretation. Time and depth structural maps generated were used to establish the structural architecture/geometry of the prospect area of the field. The depth structure map revealed NE-SW trending anticlinal structures with F5 and F6 as faults assisted closures to the reservoir. Furthermore, reservoir parameters such as net pay, water saturation porosity, net-to-gross etc, were derived from the integration of seismic and well log data. The structural interpretation on the 3-D seismic data of the study area revealed a total of seven faults ranging from synthetic to antithetic faults. The petrophysical analysis gave the porosity values of the reservoir Sand_A ranging from 18.1 - 20.3% and reservoir Sand_B ranging from 13.1-14.9% across the reservoir. The permeability values of reservoir Sand_A ranging from 63-540md and reservoir Sand_B ranging from 18-80md hence there is decrease in porosity and permeability of the field with depth.The net-to-gross varies from 22.1% to 22.4% in Rerservoir Sand A to between 5.34- 12% for Rerservoir Sand _A while Sw values for the reservoirs ranges from 38-42% in well 2 to about 68.79-96.06% in well 11. The result of original oil in place for all the wells calculated revealed that well 2 has the highest value with 9.3mmbls. These results indicate that the reservoirs under consideration have a poor to fair hydrocarbon (oil) prospect.
3D Geomechanical reservoir model for Appraisal and Development of Emi-003 field In Niger Delta, Nigeria
(Ajast, 2018-12) Lawson-Jack, Osaki; Uko, Etim D.; Opara, Alex
In this paper, geomechanical parameters were effectively integrated in 3-D geostatic model of Emi-003 reservoir in the Niger Delta basin, Nigeria for deformability and rock strength appraisal using well logs and 3D seismic volume. Unconsolidated sandstone and compacted shale were delineated and evaluated by determined elastic moduli (Poisson ratio, Young modulus, Bulk modulus, Shear modulus and Compressibility) and the Unconfined compressive strength (UCS) using sonic logs and petrophysical analysis, correlations and cross plots for comparison of the evaluated reservoir strength, physical properties (such as modulus, porosity, velocity) of the five mapped zones from five vertical wells in the studied reservoir for validation were done. Finally, incorporation of elastic properties, unconfined compressive strength in 3D static model of the studied reservoir was carried out to capture strong lateral variance of rock elastic moduli and strength into areas where well control may not exist. especially off the well points. The results show average parameters of the weakly cemented sand to have lower Poisson ratio, Young, Bulk, Shear modulus and Unconfined compressive strength (0.27, 2.3GPa, 10.8GPa, 6.91GPa, 14.21MPa respectively,) high compressibility and porosity (0.13 GPa-1, 0.26) conversely the compacted shale have higher Poisson ratio, Young, Bulk, Shear modulus and rock strength as (0.36, 8.91GPa, 18.05GPa, 21.09GPa, 56.44MPa respectively) lower compressibility and porosity (0.05 GPa-1, 0.05 respectively). There is a marked increase of rock strength and elastic moduli with relative decrease in porosity. The mechanical failure in the NNW direction of the reservoir will be relatively lower than other areas as analyse using the 3D earth model. The information gathered will help manage reservoir stress and strain induced during development and maximize reservoir performance, while mitigating risk.
Academia-industry interactions in Nigeria pharmaceutical innovation system
(Elsevier, 2012-01-01) Siyanbola, W. O.; Oladipo, O. G.; Oyewole, A. A.; Famurewa, Akindele J.; Ogundari, I. O.
This study examined the types, nature and intensity of academia-industry interactions in Nigeria’s pharmaceutical innovation system. Eight (8) top-ranked universities offering Pharmacy as a course of study, 2 Pharmaceutical Research Institutes and 25 pharmaceutical firms were sampled for the study. Interactions are predominantly in the form of knowledge flow and consultancy, staff exchange/fellowship programmes as well as sponsored workshop participations. Intensity of interactions is limited as only 20% of pharmaceutical researchers from Universities and 7% from Research Institutes had strong interactions with Pharmaceutical firms, while only 4 firms have strong interactions with the researchers.
Acute administration of co-artesiane induces oxidative stress in the testes of adult male Wistar rats
(Bioscience Research Communications, 2010) Tijani, Abiola S.; Ukwenya, Victor O.; Sodunke, Grace A.; Fakunle, Julius B.
Co-artesiane is an artemether-lumefantrine combination therapy that is used in the treatment of malaria. In this work we studied the toxicological effect of this drug with reference to seminal and biochemical parameters of the male reproductive system of wistar rats. Twenty (20) adult male rats were divided into four groups of five rats per group. Group A was administered a single dose (4 mg/kg/bw) of Co-artesiane, Group B was administered double dose (8 mg/kg/bw) of Co-artesiane and Group C received 10 mg/kg/bw of the drug for a period of 3 days. Group D served as control and received physiologic saline. In each group body weight, testicular weight, sperm count, motility and viability as well as oxidative stress status were assessed by evaluating the activities of reduced glutathione, Glutathione S-transferase, catalase, super oxide dismutase (SOD) and malondialdehyde (MDA). The mean sperm count, motility and viability in rats treated with Co-artesiane were reduced when compared with controls. Biochemical analyses showed increase in the activities of oxidative stress markers in a dose-dependent manner. The results suggest that the graded dose of Co-artesiane® elicit depletion of antioxidant defense system and induced oxidative stress in the rats.
Acute Toxicity and Lethality of Gladiolous psittacinus
(IOSR Journal of Pharmacy and Biological Sciences, 2017-04-30) Moshood, Abdullah Ishaq; Ola-Mudathir, Fausat Kikelomo; Efere, Martins Obuotor; Abdulrahim, Marufat Iyaboale; Wasiu, Idris Adegbite
The use of Gladiolouspsittacinusplant in traditional medicine is drastically increasing across the country Nigeria. This study was therefore conducted to evaluate the safety level of G. psittacinus using acute toxicity test (determination of median Lethal Dose; LD50) and the Brine shrimp lethality assay (BSLA). Histopathological analysis of the liver of rats orally administered with different dosages of G. psittacinus (1600, 2800 and 5000 mg/kg body weight) was also conducted. Results revealed amedian lethal dose (LD50) of the aqueous extract of G. psitticanus as 2116.60 mg/kg body weight. Similarly, the lethality concentration (LC50) of methanolic extract of G. psitticanus (16.950μg/ml) was higher than the test standard, K2Cr2O7 (5.653 μg/ml). The degree of lethality was also observed to be directly proportional to the concentration of the extracts. Histological studies also revealed that oral administration of G. psittacinus at 1600, 2800 and 5000 mg/kg body weight has the potential to cause some damages on the liver cells. Although G. psittacinus is a potential ethnomedicinal plant, its oral consumption is slightly toxic.
Adaptive neuro-fuzzy inference system (ANFIS) approach for the irreversibility analysis of a domestic refrigerator system using LPG/TiO2 nanolubricant
(Energy Reports, 2020-05-28) Gill, Jatinder; Singh, Jagdev; Ohunakin, Olayinka S.; Adelekan, Damola S.; Atiba, Opemipo E.; Nkiko, Mojisola O.; Atayero, Aderemi A.
This work presents an adaptive neuro-fuzzy inference system (ANFIS) artificial intelligence methodology of predicting the 2nd law efficiency and total irreversibility of a refrigeration system running on LPG/TiO2–nano-refrigerants. For this purpose, substractive clustering and grid partition approaches were utilized to train the ANFIS models required in estimating the 2nd law efficiency and total irreversibility using some experimental data. Furthermore, predictions of ANFIS models with subtractive clustering approach was found to be more accurate than ANFIS models predictions with grid partition approach. The predictions of ANFIS models with subtractive clustering approach were also compared with experimental results that were not included in the model training and predictions of already existing ANN models of authors previous publication. The comparison of variance, root mean square error (RMSE), mean absolute percentage error (MAPE) were 0.996–0.999, 0.0296–0.1726 W and 0.108–0.176 % marginal variability values. These results indicate that the ANFIS model with subtractive clustering approach having cluster radii 0.7 and 0.5 can predict the 2nd law efficiency and total irreversibility respectively, with higher accuracy than authors’ previous publication ANN models.
Adoption Kinetics and Thermodynamics of Malachite Green Onto Chitosan / Sodium Citrate Beads
(Ife Journal of Science, 2013) Bamgbose, Janet; Bamigbade, A.A.; Nkiko, Mojisola O.
Adsorption isotherms, kinetics and thermodynamic studies of methylene blue dye removal using Raphia taedigera seed activated carbon
(Caspian Journal of Environmental Science, 2020-04-30) Olasehinde, Emmanuel F.; Abegunde, Segun M.; Adebayo, Matthew A.
This present work revealed the isotherm, kinetic, and thermodynamic behaviour of methylene blue (MB) dye adsorbed onto acidic activated carbon (AAC) and base activated carbon (BAC) prepared from Raphia taedigera seed by carbonization and chemical activation. AAC and BAC were activated with sulphuric acid and sodium hydroxide respectively. Batch equilibrium studies were done under different experimental conditions such as MB dye concentration and temperature. The equilibrium data were modelled using Langmuir, Freundlich, Elovich, Temkin and Dubinin-Radushkevich isotherms. The Langmuir isotherm model best describes the uptake of MB dye onto AAC and BAC with R 2 > 0.998 in all cases. The pseudo-first-order, pseudo-second-order and intraparticle diffusion equations were used to evaluate the kinetic properties. It was observed that the adsorption of MB dye onto the two activated carbons could best be described by the pseudo-second order equation with 0.999 < R 2 ≤ 1. Thermodynamic parameters such as Gibbs free energy (ΔG0 ), standard enthalpy (ΔH0 ), standard entropy (ΔS0 ), and activation energy (Ea) were determined. The results of ΔG0 indicated a spontaneous and feasible for AAC and non-spontaneous but feasible for BAC. Results of ΔH0 confirmed that the adsorption of MB onto AAC and BAC are endothermic and physical in nature. It can be concluded that AAC and BAC prepared from Raphia taedigera seed could be used as low-cost adsorbent for the removal of MB dye from the wastewater.
Adsorption of Methylene Blue onto Acid Modified Raphia Taedigera Seed Activated Carbon
(Advanced Journal of Chemistry, 2020-04-19) Olasehinde, Emmanuel Folorunso; Abegunde, Segun Michael
In this work, the possibility of using Raphia taedigera seed as a low-cost adsorbent to remove methylene blue (MB) dye from aqueous solutions was investigated. The activated carbon was prepared by carbonization and chemical treatment of the seed with sulphuric acid to produce acidmodified Raphia taedigera seed activated carbon (RTAC). The surface morphology and bond arrangement of the RTAC were evaluated using the scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively. The SEM analysis results revealed that the prepared adsorbent had aggregated and rough surface structure with pores and FTIR revealed the presence of several functional groups such as –C=O, –OH, –C=C–, –N=O and –C=N–. The adsorption efficiency of the RTAC for the removal of the MB dye was assessed under different experimental conditions including, contact time, temperature, pH, adsorbent dosage, and dye initial concentration. The results gave the performances of 99.611% after 15 min, 99.635% at 303 K, 99.894% at pH 9, 99.256% with 0.9 g, and 99.743% for 40 mg/L for evaluating the effect of the contact time, temperature, pH, adsorbent dosage, and dye solutions initial concentration, respectively. The adsorption of the MB dye onto the RTAC conformed to Langmuir isotherm and fit best to pseudo-second-order kinetic models. Thermodynamic studies indicated a spontaneous, endothermic, feasible, and physisorption nature of the RTAC and MB dye interactions. The present study found the acid-modified Raphia taedigera seed activated carbon to be a promising low-cost adsorbent for the removal of MB dye from aqueous solution.
Alkaloid extracts from Bitter leaf (Vernonia amygdalina) and Black nightshade (Solanum nigrum) inhibit phosphodiesterase‐5, arginase activities and oxidative stress in rats penile tissue
(Wiley, 2019-04-11) Omojokun, Olasunkanmi S.; Famurewa, Akindele J.; Jaiyeoba, Oluwademilade A.; Oboh, Ganiyu; Agbebi, Oluwaseun J.
The erectogenic potential of alkaloids extracted from Bitter leaf (Vernonia amygdalina) and Black nightshade (Solanum nigrum) was investigated in this study. Fresh leaves obtained from Bitter leaf and Black night shade were air‐dried, pulverized, and extracted for alkaloids. The inhibitory potential of the alkaloid extracts on arginase and phosphodiesterase‐5 (PDE‐5) activities in rats penile tissue was determined in vitro. The antioxidant properties were also evaluated and the constituent alkaloids quantified using GC‐MS. The alkaloid extracts inhibited arginase (0–30.51 μg/ml) and PDE‐5 (0–133.69 μg/ml) activities in a concentration‐dependent pattern. Similarly, the alkaloid extracts inhibited Fe2+‐induced lipid peroxidation in rats penile tissues, scavenged DPPH, OH, and NO radicals as a function of concentration. GC‐MS characterization revealed over 20 alkaloid compounds. The inhibition of PDE‐5‐, arginase‐, pro‐oxidant‐induced lipid peroxidative‐, and free radicals‐scavenging activities by the alkaloids is suggestive of putative mechanisms underlying their therapeutic use for managing erectile dysfunction in folklore medicine. Practical applications Alkaloids extracted from Black nightshade (Solanum nigrum) and Bitter leaf (Vernonia amygdalina) were characterized and investigated by standard procedures for inhibitory action against key erectile dysfunction‐linked enzymes and antioxidant activity. The alkaloids inhibited erectile dysfunction‐linked enzymes (arginase and PDE‐5) and showed considerable antioxidant activity in a concentration‐dependent manner. In view of this, we suggest the application of these results in the development of erectile dysfunction drugs in the pharmaceutical industry, with probable minimal or no adverse effect.
Analysis of Decomposing Wood Wastes in the Lagos Lagoon using Terminal-Restriction Fragment Length Polymorphism and Illumina Sequencing Platform
(NIGERIAN JOURNAL OF TECHNOLOGICAL DEVELOPMENT, 2022-06) Buraimoh, O. M.; Adewumi, G. A.; Akinyemi, N. M.; Amund, O. O.; Ilori, M. O.; Michel Jr., F. C.
Indiscriminate disposal of wood shavings and sawdust into the Lagos lagoon usually constitute environmental hazard and varying degree of threats to marine biotic communities. In this study we applied terminalrestriction fragment length polymorphism (T-RFLP) and illumina sequencing of 16S ribosomal RNA gene, to describe the microbial ecology of decomposing wood wastes in the Lagos lagoon, Nigeria. The terminal restriction fragments (TRFs) generated corresponds to over 100 bacterial genera and numerous uncultured bacterial clones. Phylogenetic analysis of the TRFs of 16S rRNA gene sequences and cultured bacterial sequences established genetic relatedness and divergent relationships. T-RFLP in combination with illumina sequencing platform identified bacterial species (Acinetobacter sp., Clostridium sp., Planctomyces sp., Escherichia coli, Pantoea agglomerans, Dyella japonica, Ochrobactrum sp., Prevotella sp., Runella slithyformis, Enterobacter aerogenes, Gordonia amicalis, Klebsiella sp., Thalassospira sp. and Serratia sp.) not previously accounted for in culture-dependent analysis. Bacterial strains such as Ruminococcus flavefaciens, Fibrobacter succinogenes, Simiduia agarivorans, Microbulbifer sp. and Cellulomonas fimi further identified in illumina sequences were found to be involved in the biodegradation of lignin and lignin derivatives. Hence, understanding of the diverse autochthonous microbiota of decomposing wood wastes in the Lagos lagoon is essential in the development of effective biotechnology programme, to tackling the menace of sawdust pollution.
Analysis of Sustainable Cassava Biofuel Production in Nigeria
(SAGE Publications, 2012-06) Ogundari, I. O.; Momodu, A. S.; Famurewa, Akindele J.; Akarakiri, J. B.; Siyanbola, W. O.
Nigeria's biofuels policy advocates the adoption of cassava as feedstock for a 10%-biofuel substitution option in Nigerian transport fuel demand. This policy option is expected to address energy security and environmental consequences of using fossil fuels as the sole source of transport energy in the country. This paper appraised the technological and economic factors necessary for achieving Nigeria's cassava-based biofuel initiative at different substitution levels of 5, 10, and 15% by the Year 2020. A multi-stage energy forecasting and project analysis framework adapted from Coate's structure for technology assessment, as well as engineering economy methodology was used for the study. Technological analysis entailed determining petrol consumption projection, R&D capability, input feedstock requirements, environmental considerations and land requirement for feedstock crop production while engineering economy analysis evaluated the economic viability of the project. The results showed that petrol consumption in Nigeria and bioethanol substitution requirements were in the range of 18,285.7 – 19,142.84 thousand tons and 914.28 (5% low demand) – 2871.43 (15% high demand) thousand tons, respectively by 2020. Cassava feedstock and landmass requirements for bioethanol production were in the range of 4.64 – 14.53 million tons and 4.08 – 12.80 thousand sq. km, respectively while carbon dioxide savings were between 1.87 – 5.89 million tons by 2020. The recovery price for cassava bioethanol was estimated to be US$ 0.74/litre . Petrol being subsidised presently is harmful to the environment though it ‘oils’ the economy. Nigeria currently subsidizes petroleum products to the tune of 28% of 2011 budget. The government plans to remove this by 2012. Thus we conclude that weighing both economic and environmental benefits of bioethanol substitution in petrol consumption in Nigeria, the study showed that bioethanol production from cassava feedstock would be both technically and economically viable, provided subsidy, which depends on political will on the side of the government, is introduced for the first ten years of its implementation.
Antibacterial activity of lactic acid bacteria isolated from fresh pepper and tomatoes against common food pathogens
(PSCI Publications, 2014-12-31) Bello, Olorunjuwon O.; Bello, Temitope K.; Fashola, Muibat O.
This study aimed to investigate the antibacterial activity of lactic acid bacteria (LAB) isolated from fresh pepper and tomatoes against common food borne pathogens. Man Rogosa Sharpe (MRS) broth and agar were used for the isolation of LAB from the food products and whose antagonistic properties were tested against Bacillus cereus, Staphylococcus aureus, Streptococcus sp., E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa using well diffusion assay method. Four LAB species were isolated and these included Streptococcus pyogenes, Enterococcus faecalis, Lactococcus casei and Lactococcus fermentii. The percentage occurrence of LAB species ranged from 11.77% to 35.29%. E. faecalis exerted the strongest antibacterial activity against all selected pathogenic bacteria while L. casei showed the weakest activity. It was concluded that the isolated LAB showed remarkable inhibitory effect against tested pathogenic strains. It is therefore suggested that these potent isolates could be used as a natural bio-preservatives in different food products. However, further in vitro and in vivo studies are required, according to selection criteria, on their application in different food products.
Antibiogram of bacterial flora of public health significance associated with postharvest Irvingia gabonensis seeds in Lagos State, Nigeria
(The Proceedings of the Nigerian Academy of Science, 2020-08-02) Bello, Olorunjuwon Omolaja; Bello, Temitope Kudirat; Babalola, Olubukola Oluranti; Olu-Aderoumu, William Olusola
The spread of resistant bacteria within the community has continually posed obvious additional problems for infectious control. Efforts to identifying sources of resistant bacteria have not been channeled towards medicinal food condiments. This study investigated the antibiogram of bacterial flora of public health significance associated with postharvest Irvingia gabonensis seeds in Lagos State, Nigeria. The blended, homogenized and serially diluted samples of I. gabonensis seeds were plated using the spread plate technique on selective and differential agar media. API 20E and API 20NE were used for identification of members of Enterobacteriaceae and non-Enterobacteriaceae, respectively. The agar disc-diffusion method was employed to determine the antibiotic resistance profiles of the isolates. A total of 263 bacterial isolates (129 Gram-positive and 134 Gram-negative) were encountered. Eighty-five (66%) of Gram-positive isolates exhibited resistance to penicillin, gentamicin 65 (50.4%), erythromycin 69 (53.5%), cloxacillin 63 (43.8%), chloramphenicol 73 (56.6%), amoxicillin 75 (58.1%), tetracycline 58 (45%) while 69 (53.5%) showed resistance to streptomycin. However, 87 (65%) of Gram-negative bacterial strains exhibited resistance to cloxacillin, ceftazidime 82 (61.2%), ciprofloxacin 67 (50%), gentamicin 77 (57.5%), cefotaxime 74 (55.2%), augmentin 84 (62.7%), nitrofuratoin 61 (45.5%) and 24 (17.9%) to ofloxacin. This study showed that I. gabonensis seeds could be a source of antibiotic-resistant bacterial strains, despite its enormous medicinal properties, which is a threat to public health. The antibiotic resistance patterns of isolated bacterial strains are of medical importance as there are chances of transferring resistant traits.
Antibiotic Susceptibility Profiles and Bacteriological Risks Associated With Used Toothbrushes: A Case Study of Some Apparently Healthy University Students in Southwestern Nigeria
(American Research Institute for Policy Development, 2013-07-30) Bello, O. O.; Osho, A.; Bankole, S.A.; Bello, T. K.
Toothbrushes play a significant role in disease transmission and increase the risk of infection since they serve as reservoirs for microorganisms in healthy, oral-diseased and medically ill adults. Investigation was carried out on the antibiotic susceptibility profiles of bacteria isolated from used toothbrushes. Thirty toothbrushes used for at least 5 weeks by thirty University students were collected. Heads of the brushes were soaked in 10 ml of sterile tryptone soya broth (TSB) and agitated by vortex mixing. The bacterial suspension was serially diluted. Plate count agar, MacConkey agar and Mannitol salt agar media were used for the isolation of non-fastidious bacteria, coliforms and staphylococci, respectively, employing the spread plate technique. Biochemical characterization of isolates was carried out using standard methods. Survival ability of bacterial contaminants on the used toothbrushes was also investigated at the 24th hr, 72nd hr and 144th. The disk diffusion method was employed for the determination of the antimicrobial susceptibility profiles of the bacterial isolates. Seven genera of microorganisms were encountered and these include Staphylococcus, Escherichia, Klebsiella, Pseudomonas, Lactobacillus, Leuconostoc and Proteus. Pseudomonas aeruginosa was most prevalent as shown by mean total plate count of 5.0 x 102 CFU ml-1 while E. coli had the lowest prevalence (1.2 x 102 CFU ml-1). It was discovered that S. aureus, S. epidermidis, E. coli and Proteus sp all survived at 144th hr indicating high survival ability, while Lactobacillus sp only survived at 24th hr. There were variations in the susceptibility patterns of the isolates to the various antibiotics. It was determined that 62.5% of the isolates showed susceptibility; twenty percent (20%) of isolates were intermediately susceptible and the remaining 17.5% were resistant. It was concluded that most bacterial isolates from toothbrushes were susceptible to antibiotics but the percentage resistant should be of great concern as it poses high health risk and may generate the spread of antibiotic-resistant bacteria within the family and beyond. Organisms such as some members of the enterobacteriaceae which are not normally associated with oral flora isolated from used toothbrushes investigated in this study should also be of interest.
Antibiotic Susceptibility Profiles of Bacteria from Diabetic Foot Infections in Selected Teaching Hospitals in Southwestern Nigeria
(AIJR Publisher., 2018-01-28) Bello, Olorunjuwon O.; Oyekanmi, Edward O.; Kelly, Babatunde A.; Mebude, Olakunle O.; Bello, Temitope K.
One of the serious complications associated with diabetes is foot ulcer and this condition affects the quality of life in patients in all classes, races and ages. Chronic wounds are prone to colonization by wide array of microorganisms which could be extremely hazardous to patients if effective and timely therapeutic intervention is not made. This study was conducted to determine the antibiotic susceptibility profiles of bacteria from diabetic foot infections in southwestern Nigeria. Samples were collected from a total of 142 diabetic foot ulcer patients with moistened sterile cotton swabs. Nutrient agar, Mac-Conkey agar, blood agar and mannitol salt agar media were used for the isolation of total viable bacteria, Gram-negative non-spore forming lactose fermenters, fastidious bacteria and staphylococci, respectively. Morphological and biochemical characteristics of pure cultures were determined in accordance with standard laboratory criteria. API 20 E and API 20 NE were used for the confirmation of identity of the bacterial isolates. The disc diffusion technique was employed for the determination of antibiotic susceptibility of bacterial isolates in accordance with standard procedures. The antibiotics investigated included amikacin, amoxicillin, ampicillin, ceftazidime, cefazolin, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, gentamicin, imipenem, linezolid, methicillin, netilmicin, ofloxacin, oxacillin, penicillin, piperacilin, sulfamethoxazole, trimethoprim and vancomycin. One hundred and seventy-seven isolates were encountered and these were characterized into eleven bacterial species. These included Staphylococcus aureus (22.03%), Pseudomonas aeruginosa (16.95%), Staphylococcus epidermidis (12.43%), Proteus mirabilis (8.48%), Klebsiella pneumoniae (7.91%), E. coli (7.35%), Staphylococcus saprophyticus (6.78%), Streptococcus pyogenes (5.65%), Morganella morganii (5.09%), Citrobacter freundii (4.52%) and Acinetobacter baumannii (2.83%). Gram-negative bacteria showed 76.99% susceptibility to the antibiotics while 22.85% was resistant. Gram-positive bacteria showed 93.75% susceptibility and 5.01% resistance to the antibiotics. This study revealed that there is no definite aetiologic bacterial agent for diabetic foot infections and many of the associated bacteria are sensitive to certain antibiotics.
ANTIBIOTICS RESISTANCE PROFILE OF ESCHERICHIA COLI AND ENTEROBACTER AEROGENES ISOLATED FROM WELL WATERS IN AGO-IWOYE, SOUTHWESTERN NIGERIA
(Council for Innovative Research, 2013-12-01) Bello, Temitope K.; Bello, Olorunjuwon O.; Egberongbe, H.O.; Azeez, Ikimot A.; Oluwadun, Afolabi
Eighty percent of all diseases are attributed to unsafe water with about 11.4 billion people in the world suffering from major related diseases at various times. This study was carried out to investigate the antibiotics resistance profile of Escherichia coli and Enterobacter aerogenes isolated from well waters in Ago-Iwoye, Southwestern Nigeria. Water samples were collected from ten different wells. The multiple tube fermentation technique was employed to enumerate coliforms using MacConkey broth. Nutrient agar and ethylene methylene blue (EMB) agar were used for the enumeration of Escherichia coli and Enterobacter aerogenes. Biochemical characterization was carried out using standard methods. The disk diffusion method was used to determine the antibiotics susceptibility profiles of the bacterial isolates. Results showed that wells contained most probable number (MPN) of bacteria ranging from 43 to 1,100 bacteria per 100 ml. E. coli was present in eight of the wells while E. aerogenes was present in all the wells. Wells B and C had the highest incidence of E. coli with 5.0 x 102 CFU/100ml while wells D and J had no incidence of E. coli. Occurrence of Enterobacter aerogenes was highest in well B (4.5 x102 CFU/100ml), followed by well C (4.0 x102 CFU/100ml) while the lowest occurrence was obtained from well F having a count of 2.0 x 102 CFU/100ml. E. coli was resitant to nitrofurantoin, ampicillin, cephalocidine, sulphafurazole, carbenicillin and sulfamethazole while E. aerogenes was sensitive to colistin, gentamicin and nitrofurantoin but resistant to the remaining antibiotics of the Gram negative disc. For the U4 disc, E. coli was susceptible to colistin sulphate and resistant to all other antibiotics. E. aerogenes was resistant to the entire U4 discs. The presence of E. coli and E. aerogenes suggested faecal pollution, hence the quality of the wells fell strongly below the standard of safe drinking water. Most strains of isolates showed relative resistance to antibiotics investigated in this study and this should be of great concerns to researchers.
Antihyperlipidaemic and Antioxidant Potential of Fermented Citrullus vulgaris Seeds (Thunb.) on Tyloxapol-induced Hyperlipidaemic Rats: A Comparison With Fluvastatin
(Canadian Center of Science and Education, 2015-08-01) Ayo-Lawal, Aderonke R.; Osoniyi, Omolaja; Famurewa, Akindele J.
Globally fermented foods form an integral part of the staple diet of people. Fermented Citrullus vulgaris (FCV) (ogiri) seeds is a nutritious natural fermented condiment that features frequently in West African diets as a spice.This study investigated the antihyperlipidaemic potential of this condiment in tyloxapol-induced hyperlipidaemic rats with reference to that of fluvastatin, a standard antilipidaemic drug. The antioxidant potential of the condiment was also examined using different relevant in vitro assays. Albino rats were divided into six groups (n=5), based on the diet and treatment received. The groups were standard feed (control); FCV supplemented feed; standard feed with tyloxapol administered at the end of the experimental period (tyloxapol control); FCV supplemented feed and tyloxapol at the end of the experimental period; standard feed with fluvastatin sodium (40 mg/kg and 80 mg/kg body weight separately) followed by tyloxapol. The results revealed that administration of tyloxapol induced significant (p < 0.05) increase in total-cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and triglyceride (TG). These lipid increases were significantly mitigated in groups initially pre-fed with supplemented FCV feed. Plasma TC decreased by 69.38% (p < 0.05); TG by 80.58% (p < 0.05); LDL-C by 7.80% (p < 0.05) and high density lipoprotein cholesterol (HDL-C) levels increased by 78.61% (p < 0.05). FCV showed appreciable antioxidant activities in vitro in a dose dependent manner. Histomorphological examination of the liver suggested that the FCV possessed hepatoprotective potential. These results suggest that FCV consumption may be a possible dietary measure for the control of dyslipidaemia.
Antioxidant activities and glycemic indices of ice creams enriched with orange (Citrus sinensis) and shaddock (Citrus maxima) peels and effects on rat lipid profiles
(Wiley, 2021-05) Ademosun, Ayokunle Olubode; Oboh, Ganiyu; Ajeigbe, Olufunke Florence
This study evaluated the effects of ice creams produced from blends of orange (Citrus sinensis) and shaddock (Citrus maxima) peels on the blood lipid profile, glycemic index, and antioxidant indices in the liver and heart of rats. Formulated ice cream was produced at a different proportion of citrus (orange and shaddock) blends and fed to rats for 28 days. The result showed that the formulated ice cream enriched with citrus peels blends caused a significant increase in high-density lipoprotein-cholesterol level in the plasma and antioxidant status in the liver and heart homogenates, decreased the glycemic index, concentration of total cholesterol, triglycerides, and low-density lipoprotein in the plasma as against rats fed on plain and commercial ice creams. To conclude, the use of ice creams from blends of orange and shaddock peels could serve as a functional food for weight reduction, glycemic index, management of lipid-related diseases, and prevention of oxidative stress-related complications in the liver and heart. Practical applications The consumption of ice creams has increased in many parts of the world. However, there have been limited efforts aimed at improving the medicinal properties of frozen dairy products. Hence, these ice creams could be produced on a large scale under regulated condition since they have improved medicinal properties which would be a good option for preventing/combating degenerative conditions and their related complications.
Antioxidant activities and glycemic indices of ice creams enriched with orange (Citrus sinensis) and shaddock (Citrus maxima) peels and effects on rat lipid profiles
(Wiley, 2021-05) Ademosun, Ayokunle Olubode; Oboh, Ganiyu; Ajeigbe, Olufunke Florence
This study evaluated the effects of ice creams produced from blends of orange (Citrus sinensis) and shaddock (Citrus maxima) peels on the blood lipid profile, glycemic index, and antioxidant indices in the liver and heart of rats. Formulated ice cream was produced at a different proportion of citrus (orange and shaddock) blends and fed to rats for 28 days. The result showed that the formulated ice cream enriched with citrus peels blends caused a significant increase in high-density lipoprotein-cholesterol level in the plasma and antioxidant status in the liver and heart homogenates, decreased the glycemic index, concentration of total cholesterol, triglycerides, and low-density lipoprotein in the plasma as against rats fed on plain and commercial ice creams. To conclude, the use of ice creams from blends of orange and shaddock peels could serve as a functional food for weight reduction, glycemic index, management of lipid-related diseases, and prevention of oxidative stress-related complications in the liver and heart. Practical applications The consumption of ice creams has increased in many parts of the world. However, there have been limited efforts aimed at improving the medicinal properties of frozen dairy products. Hence, these ice creams could be produced on a large scale under regulated condition since they have improved medicinal properties which would be a good option for preventing/combating degenerative conditions and their related complications.

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