Biochemical characterization and kinetic studies on a purified yellow laccase from newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decayed plant matter

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dc.contributor.authorAdemakinwa, Adedeji N.
dc.contributor.authorAgboola, Femi K.
dc.date.accessioned2019-08-14T14:16:23Z
dc.date.available2019-08-14T14:16:23Z
dc.date.issued2016-06
dc.description.abstractThe study investigated the biochemical characteristics and kinetic parameters of laccase from a newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decay plant litters. This was with a view to identifying the type of laccase and its possible suitability for biotechnological applications. The fungal strain was identified as A. pullulans NAC8 by sequencing of its 5.8S rRNA and adjacent internally transcribed sequences (ITS) 1 and 2. A. pullulans NAC8 laccase was purified 2.0-fold with a yield of 59.3% and specific activity of 9.34 μmol/min/mg protein. The kinetic parameters KM, Vmax, kcat and kcat/KM for laccase with guaiacol as substrate were 1.05 ± 0.12 mM, 12.67 ± 0.55 μmol/ml/min, 25.3 × 10−1 s−1 and 2.4 × 103 M−1 s−1 respectively. Laccase exhibited maximum activity at 45 °C and optimum pH of 4.5. The enzyme showed stability at a temperature range of 45–55 °C after a 2 h incubation. The molecular weight determined on SDS–PAGE was 68.4 kDa. The enzyme was stable at 10% of all organic solvents used but displayed a loss of activity at 50%. 2.5 mM thioglycolic acid (TGA) and 0.05 mM sodium azide inactivated the enzyme. The substrate specificity was guaiacol > catechol > tannic acid > gallic acid. There was no peak observed at 610 nm and the ratio of absorbance at 280 nm and 610 was 26. This suggests a yellow laccase. The biochemical properties of A. pullulans NAC8 yellow laccase makes it potentially useful in several biotechnological applicationsen_US
dc.description.sponsorshipN/Aen_US
dc.identifier.citationAdemakinwa, A. N., & Agboola, F. K. (2016). Biochemical characterization and kinetic studies on a purified yellow laccase from newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decayed plant matter. Journal of Genetic Engineering and Biotechnology, 14(1), 143-151.en_US
dc.identifier.uri10.1016/j.jgeb.2016.05.004
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/472
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.subjectLaccaseen_US
dc.subjectAureobasidium pullulansen_US
dc.subjectPurificationen_US
dc.subjectBiochemical Characterizationen_US
dc.titleBiochemical characterization and kinetic studies on a purified yellow laccase from newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decayed plant matteren_US
dc.typeArticleen_US
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