Fungal α-amylase entrapped in agar-agar organic matrix “beads” enhances fabric starch-desizing potentials and α-amylase-detergent compatibility

Simple item page
dc.contributor.authorAdemakinwa, Adedeji Nelson
dc.contributor.authorAyinla, Zainab Adenike
dc.contributor.authorAgboola, Femi Kayode
dc.date.accessioned2021-11-08T11:03:49Z
dc.date.available2021-11-08T11:03:49Z
dc.date.issued2021-08-05
dc.descriptionStaff Publicationen_US
dc.description.abstractAureobasidium pullulans α-amylase (ApAmy) mixed with melted agar-agar solution and drop-wisely added to a mixture of organic solvent solution allowed for the entrapment of the α-amylase in the agaragar organic matrix as beads. The immobilized ApAmy’s characteristics and wash performance were elucidated in comparison with the soluble ApAmy. Agar-agar at 2.0 % (w/v) and toluene: chloroform at 3:1 resulted in the highest immobilization yield retaining about 98% residual activity after ten catalytic cycles. The optimum temperature and pH for the immobilized enzyme were 60ºC and 6.5 respectively. The immobilized ApAmy hydrolysed branched and linear substrates thus establishing its broad substrate specificity. Relatively, the immobilized ApAmy (iApAmy) was more tolerant to organic solvents than the free enzyme. The iApAmy was mildly inhibited by cobalt but metals such as zinc, manganese, calcium and sodium enhanced the free and immobilized ApAmy activity. The iApAmy had a higher washing efficiency (77%) in the presence of detergents than the free enzyme (68%) and control (36%). The iApAmy showed good potentials as a detergent additive and from its characteristics, it could be useful in other industrial applications.en_US
dc.identifier.urihttps://doi.org/10.21203/rs.3.rs-783369/v1
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/1293
dc.language.isoenen_US
dc.publisherResearch Squareen_US
dc.subjectα-amylase,en_US
dc.subjectAureobasidium pullulans,en_US
dc.subjectagar-agar,en_US
dc.subjectimmobilization,en_US
dc.subjectdetergentsen_US
dc.titleFungal α-amylase entrapped in agar-agar organic matrix “beads” enhances fabric starch-desizing potentials and α-amylase-detergent compatibilityen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
16ccb4c5-a2f3-4ec9-ae81-e66c367eab95.pdf
Size:
514.09 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles Biological Sciences