Purification and biochemical characterization of a β-cyanoalanine synthase expressed in germinating seeds of Sorghum bicolor (L.) moench

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dc.contributor.authorAmiola, Ruth Ololade
dc.contributor.authorAdemakinwa, Adedeji Nelson
dc.contributor.authorAyinla, Zainab Adenike
dc.contributor.authorEzima, Esther Nkechi
dc.contributor.authorAgboola, Femi Kayode
dc.date.accessioned2021-02-17T11:55:09Z
dc.date.available2021-02-17T11:55:09Z
dc.date.issued2018-02-06
dc.descriptionStaff Publicationen_US
dc.description.abstractBackground: β-Cyanoalanine synthase plays essential roles in germinating seeds, such as in cyanide homeostasis. Methods: β-Cyanoalanine synthase was isolated from sorghum seeds, purified using chromatographic techniques and its biochemical and catalytic properties were determined. Results: The purified enzyme had a yield of 61.74% and specific activity of 577.50 nmol H2S/min/mg of protein. The apparent and subunit molecular weight for purified β-cyanoalanine synthase were 58.26 ± 2.41 kDa and 63.4 kDa, respectively. The kinetic parameters with sodium cyanide as substrate were 0.67 ± 0.08 mM, 17.60 ± 0.50 nmol H2S/mL/min, 2.97 × 10−1 s−1 and 4.43 × 102 M−1 s−1 for KM, Vmax, kcat and kcat/KM, respectively. With L-cysteine as substrate, the kinetic parameters were 2.64 ± 0.37 mM, 63.41 ± 4.04 nmol H2S/mL/min, 10.71 × 10−1 s−1 and 4.06 × 102 M−1 s−1 for KM, Vmax, kcat and kcat/KM, respectively. The optimum temperature and pH for activity were 35°C and 8.5, respectively. The enzyme retained more than half of its activity at 40°C. Inhibitors such as HgCl2, EDTA, glycine and iodoacetamide reduced enzyme activity. Conclusion: The biochemical properties of β-cyanoalanine synthase in germinating sorghum seeds highlights its roles in maintaining cyanide homeostasisen_US
dc.identifier.citationAmiola, R.O., Ademakinwa, A.N., Ayinla, Z.A., Ezima, E.N., & Agboola, F. (2018). Purification and biochemical characterization of a β-cyanoalanine synthase expressed in germinating seeds of Sorghum bicolor (L.) moench. Turkish Journal of Biochemistry, 43, 638 - 650. https://doi.org/10.1515/tjb-2017-0214en_US
dc.identifier.issn1303-829X
dc.identifier.urihttps://doi.org/10.1515/tjb-2017-0214
dc.identifier.urihttp://repository.elizadeuniversity.edu.ng/handle/20.500.12398/877
dc.language.isoenen_US
dc.publisherDe Gruyter : Turkish Journal of Biochemistryen_US
dc.subjectβ-Cyanoalanine synthase;en_US
dc.subjectCyanide;en_US
dc.subjectSorghum;en_US
dc.subjectDetoxification;en_US
dc.subjectCharacterization;en_US
dc.subjectPurification.en_US
dc.titlePurification and biochemical characterization of a β-cyanoalanine synthase expressed in germinating seeds of Sorghum bicolor (L.) moenchen_US
dc.typeArticleen_US
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